We narrowed to 9,586 results for: Coli

Showing: 2351 - 2400 of 9586 results

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pSG 853

Plasmid

#63873

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAA near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 163

Plasmid

#63871

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pBbE2k-pTet-gRNAwcaF159

Plasmid

#122259

Purpose

aTc inducible gRNA expression plasmid

Depositor

Chueh Loo Poh

Article

Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

Insert

gRNAwcaF159

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPmal

Plasmid

#14478

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Tags

ECFP and EYFP

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-P41nmt1-3FLAG

Plasmid

#19349

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

hphMX6

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFLIPmal-40uDelta1Venus

Plasmid

#18854

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

pFLIPmal-40uDelta1V

Tags

CFP and Venus

Expression

Bacterial

Mutation

N227A

Available Since

Oct. 23, 2008

Availability

Academic Institutions and Nonprofits only
pKP1141

Plasmid

#160811

Purpose

Expression of TonB protein

Depositor

Kathleen Postle

Article

Gresock et al Front Microbiol. 2011 Oct 12;2:206. doi:

Insert

tonB (tonB Escherichia coli)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-P3nmt1-3FLAG

Plasmid

#19348

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

hphMX6

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pSelect-2

Plasmid

#190242

Purpose

Encodes lacI-targeting gRNA for positive selection of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMON-HsNMT2

Plasmid

#67476

Purpose

Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRB30

Plasmid

#165480

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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circRAJ31

Plasmid

#69926

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-circRAJ31

Use

Synthetic Biology

Expression

Bacterial

Promoter

PltetO1-2

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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FLIPrbs F16A

Plasmid

#17864

Depositor

Wolf Frommer

Article

Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

Insert

FLIPrbs F16A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pNK141k2

Plasmid

#156384

Purpose

Expresses orthogonal RBS mCarmine reporter

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

mCarmine

Use

Synthetic Biology

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD24MevT

Plasmid

#17820

Depositor

Jay Keasling

Article

Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

Insert

MevT

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPmal-25u

Plasmid

#14480

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W230A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pNK141f2

Plasmid

#156379

Purpose

Expresses orthogonal RBS Venus reporter

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

Venus

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTK203

Plasmid

#13536

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-TCTY

Tags

See map

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pBbE7c-XAacxnT (JBEI-14118)

Plasmid

#100961

Purpose

XAacx operon in pBbE7c

Depositor

Jay Keasling

Article

Brown et al ACS Synth Biol. 2016 Dec 16;5(12):1485-1

Insert

XAacx operon

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
No-repeats

Plasmid

#122487

Purpose

A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Insert

LacZ

Expression

Insect

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FLIPrbs Q235A

Plasmid

#17862

Depositor

Wolf Frommer

Article

Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

Insert

FLIPrbs Q235A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

Q235A

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pKB253

Plasmid

#170036

Purpose

Encodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
K5L7

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

May 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFF828

Plasmid

#61456

Purpose

AHL-inducible msd_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_lacZ(ON)

Use

Synthetic Biology

Promoter

pLuxR

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pN23-LIC-His-FLAG

Plasmid

#165478

Purpose

pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMMDuet19

Plasmid

#45594

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

NpuDnaB mini intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Mutation

removal of endonuclease domain

Promoter

T7

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEDuet40

Plasmid

#46378

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

TvoVMA intein

Tags

GB1 and H6-MBP

Expression

Bacterial

Promoter

T7

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
FLIPrbs F15A

Plasmid

#17863

Depositor

Wolf Frommer

Article

Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

Insert

FLIPrbs F15A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F15A

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pDL11

Plasmid

#17832

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB,

Use

Synthetic Biology; Aspergillus nidulans expression

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pRB33-GFP

Plasmid

#165483

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-GFP-TEV

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FLIPrbs R141A

Plasmid

#17865

Depositor

Wolf Frommer

Article

Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

Insert

FLIPrbs R141A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

R141A

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pAB184a

Plasmid

#156358

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPmal-225u

Plasmid

#14481

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W62A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pJK_proJK1_LGK

Plasmid

#81160

Purpose

Expresses levoglucosan kinase with promoter proJK1

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase wild-type

Tags

His6x

Expression

Bacterial

Promoter

proJK1

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLIPrbs T135A

Plasmid

#17861

Depositor

Wolf Frommer

Article

Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

Insert

FLIPrbs T135A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T135A

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pRSET FLIPmal-control

Plasmid

#14482

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W340A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pFF761

Plasmid

#61455

Purpose

aTc-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSigma54-4

Plasmid

#41748

Depositor

Jeff Gelles

Article

Friedman et al Cell. 2012 Feb 17;148(4):679-89. doi: 10

Insert

rpoN (rpoN E. coli)

Tags

n-terminal HHHHHHssClvprgsh

Expression

Bacterial

Mutation

c198a c307g c346a

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pNQ116

Plasmid

#242080

Purpose

Expresses NusG (M1-P124) as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

NusG (nusG E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

conatins M1-P124

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNQ117

Plasmid

#242081

Purpose

Expresses NusG-F165A as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

NusG (nusG E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

F165A

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNQ058

Plasmid

#242079

Purpose

Expresses rS1 as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

rpsa (rpsA E. coli)

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNQ055

Plasmid

#242077

Purpose

Expresses MetRS (truncated at K548) as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

metG (metG E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

contains M1-K548

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNQ056

Plasmid

#242078

Purpose

Expresses MTF as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

FMT (fmt E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

M1 is deleted

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK1062

Plasmid

#236186

Purpose

BsaI compatible kanamycin resistance cassette

Depositor

Jeffrey Barrick

Article

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

Kanamycin resistance cassette

Expression

Bacterial

Promoter

CP25

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DVA_p15A_AH

Plasmid

#217602

Purpose

Destination vector with ampicillin resistance and p15A origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF

Plasmid

#216326

Purpose

PUlaA promoter driving expression of sfgfp

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJEC619

Plasmid

#182955

Purpose

SYNZIP18-Cascade.

Depositor

James Chappell

Article

Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

Insert

SynZip18-Cascade

Tags

SynZip18

Expression

Bacterial

Mutation

SynZip18 fusion to CasA

Promoter

J23150

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEST-PT7extended-MGapt-UTR1-deGFP

Plasmid

#227646

Purpose

pT7-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis with extended T7 promoter.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DVK_pBR322_FG

Plasmid

#217595

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DVK_pBR322_AF

Plasmid

#217593

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 9,586 results for: Coli