-
Plasmid#127110DepositorInsertGFP-mCherry fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW228
Plasmid#113242PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationTTGGTAG->tAggtagPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW227
Plasmid#113241PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationTTGGTAG->AtggtagPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-SpAtg17(416)
Plasmid#122079PurposeExpressing S. pombe HA-Atg17DepositorInsertSpATG17 (atg17 Fission Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-SpAtg17(416)
Plasmid#122078PurposeExpressing S. pombe Atg17DepositorInsertSpATG17 (atg17 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW207
Plasmid#113247PurposeE. coli expression vector for IHFa K20A + IHFbDepositorInsertsUseTagsExpressionBacterialMutationK20APromoterT7Available sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW205
Plasmid#113245PurposeE. coli expression vector for IHFa D14A + IHFbDepositorInsertsUseTagsExpressionBacterialMutationD14APromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) short BP-3'SS
Plasmid#111333PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationshorter branch to 3' splice site distancePromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPS-1-cmyc
Plasmid#113059PurposeExpresses worm Myc-tagged MPS-1 in mammalian cellsDepositorInsertMiRP K+ channel accessory Subunit 1 (mps-1 Nematode)
UseTagsc-MycExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW217
Plasmid#113239PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggtaCPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW215
Plasmid#113237PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggAagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW214
Plasmid#113236PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->gCtagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW213
Plasmid#113235PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CgtagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW211
Plasmid#113233PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGADepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->AACGAPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW210
Plasmid#113189PurposeE. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationH208APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW216
Plasmid#113238PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggtTgPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW203
Plasmid#113243PurposeE. coli expression vector for IHFa E10A + IHFbDepositorInsertsUseTagsExpressionBacterialMutationE10APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Dhr1
Plasmid#100003PurposeExpress yeast Dhr1 with Y2H activation domain fusionDepositorInsertDhr1 (ECM16 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Dhr1
Plasmid#100002PurposeExpress yeast Dhr1 with Y2H binding domain fusionDepositorInsertDhr1 (ECM16 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Mrt4
Plasmid#99997PurposeExpress yeast Mrt4 with Y2H binding domain fusionDepositorInsertMrt4 (MRT4 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Mrt4
Plasmid#99998PurposeExpress yeast Mrt4 with Y2H activation domain fusionDepositorInsertMrt4 (MRT4 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Esf1
Plasmid#99988PurposeExpress yeast Esf1 with Y2H activation domain fusionDepositorInsertEsf1 (ESF1 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Utp15
Plasmid#99978PurposeExpress yeast Utp15 with Y2H activation domain fusionDepositorInsertUtp15 (UTP15 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Utp15
Plasmid#99977PurposeExpress yeast Utp15 with Y2H binding domain fusionDepositorInsertUtp15 (UTP15 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP757-1
Plasmid#99493Purpose6XHis::TEV::3XFlag::linker::MycDepositorInsert6XHis::TEV::3XFlag::linker::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP605-1
Plasmid#99482PurposeMyc::TEV::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::TEV::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP640-2
Plasmid#99487Purpose3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlagDepositorInsert3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Cka2
Plasmid#99980PurposeShuttle yeast Cka2 into Gateway destination vectorDepositorInsertCka2 (CKA2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Utp16
Plasmid#99985PurposeShuttle yeast Utp16 into Gateway destination vectorDepositorInsertUtp16 (BUD21 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-T_RFP entry
Plasmid#87526PurposeDestination vector for domain insertionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale empty functiona…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-A_RFP entry
Plasmid#87527PurposeDestination vector for domain insertionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale empty functiona…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-C_RFP entry
Plasmid#87528PurposeDestination vector for domain insertionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale empty functiona…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTALE-STAR-G_RFP entry
Plasmid#87529PurposeDestination vector for domain insertionDepositorTypeEmpty backboneUseSynthetic Biology and TALEN; Tale empty functiona…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
35S-frGC155-MXMT-NosT
Plasmid#80172PurposeBiFC vector for transient expression of frGC155-MXMT in plantsDepositorInsertfrGFP(156-238)
UseTagsfrGC155ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S-psGC155-MXMT-NosT
Plasmid#80171PurposeBiFC vector for transient expression of psGC155-MXMT in plantsDepositorInsertpsGFP(156-238)
UseTagspsGC155ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S-sfGC155-MXMT-NosT
Plasmid#80166PurposeBiFC vector for transient expression of sfGC155-MXMT in plantsDepositorInsertsfGFP(156-238)
UseTagssfGC155ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH011
Plasmid#80395PurposeContains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry and TetR genes. Used for Western blots.DepositorInsertBicistronic Scaffold-3xFLAG-SD-mCherry
UseSynthetic BiologyTags3xFLAG tag on scaffold protein for WesternExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S-psGN155-MXMT-NosT
Plasmid#80170PurposeBiFC vector for transient expression of psGN155-MXMT in plantsDepositorInsertpsGFP(1-155)
UseTagsMXMT and psGN155ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S-sfGN155-MXMT-NosT
Plasmid#80165PurposeBiFC vector for transient expression of sfGN155-MXMT in plantsDepositorInsertsfGFP(1-155)
UseTagssfGN155ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S-frGN155-MXMT-NosT
Plasmid#80164PurposeBiFC vector for transient expression of frGN155-MXMT in plants.DepositorInsertfrGFP(1-155)
UseTagsfrGN155ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S-eGN155-MXMT-NosT
Plasmid#80130PurposeBiFC vector for transient expression of eGN155-MXMT in plantsDepositorInserteGFP(1-155)
UseTagseGN155ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
MetNI (E166Q in MetN)
Plasmid#78320PurposeMethionine ABC TransporterDepositorInsertsMetI
MetN
UseTags10x Histidine Tag and 6x Histidine TagExpressionBacterialMutationE166QPromoterT7 PromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly(alanylglycine)
Plasmid#67003PurposeExpresses malE-poly(alanylglycine) in E. coliDepositorInsertmalE-poly(alanylglycine)
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly-EAK9
Plasmid#67010PurposeExpresses malE-poly-EAK9 in E. coliDepositorInsertmalE-poly-EAK9
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-CBPNDHFR
Plasmid#63995PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCalmodulin Binding Peptide (CBP); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HPCHISNDHFR
Plasmid#63984PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Protein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HPCHISNDHFR
Plasmid#63948PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Protein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-CBPHISNDHFR
Plasmid#63945PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Calmodulin Binding Peptide (CBP); TEV…ExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-STRHISNDHFR
Plasmid#63982PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-CBPNDHFR
Plasmid#63977PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCalmodulin Binding Peptide (CBP); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only