We narrowed to 2,507 results for: Alb

1. pET28b-PU.1-ETS-H2

   Plasmid

   #123358

   Purpose

   Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 207 to 215 replaced with corresponding s…

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. M3K5_HUMAN_D0

   Plasmid

   #79710

   Purpose

   This plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   M3K5 (MAP3K5 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. E2AK2_HUMAN_D0

   Plasmid

   #79712

   Purpose

   This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   E2AK2 (EIF2AK2 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. DMPK_HUMAN_D0

   Plasmid

   #79717

   Purpose

   This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   DMPK (DMPK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. KC1G1_HUMAN_D0

   Plasmid

   #79691

   Purpose

   This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KC1G1 (CSNK1G1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. CSK22_HUMAN_D0

   Plasmid

   #79704

   Purpose

   This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSK22 (CSNK2A2 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

   Plasmid

   #89992

   Purpose

   Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABL1_HUMAN_D0

   Plasmid

   #79727

   Purpose

   This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   ABL1 (ABL1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. FGFR1_HUMAN_D0

   Plasmid

   #79719

   Purpose

   This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   FGFR1 (FGFR1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. CHK2_HUMAN_D0

    Plasmid

    #79715

    Purpose

    This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CHK2 (CHEK2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. MK08_HUMAN_D0

    Plasmid

    #79695

    Purpose

    This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK08 (MAPK8 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pET28B-PU.1-ETS-Wing

    Plasmid

    #124552

    Purpose

    Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    The 5 residues making up the "wing" in …

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CSKP_HUMAN_D0

    Plasmid

    #79714

    Purpose

    This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSKP (CASK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MK01_HUMAN_D0

    Plasmid

    #79713

    Purpose

    This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK01 (MAPK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid

    #108362

    Purpose

    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

    Depositor

    Luisa Cochella

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Inserts

    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    

    Tags

    2xFlag, MYC, T2A peptide, and TY1 linker

    Expression

    Worm

    Promoter

    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX303 TagRFP-T LGALS3 R186S

    Plasmid

    #202428

    Purpose

    Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    TagRFP

    Mutation

    R186S

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid

    #171813

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid

    #171804

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDS2149

    Plasmid

    #184275

    Purpose

    Tet-on genome insertion system

    Depositor

    Dominique Sanglard

    Insert

    CatrTA
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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