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pPRISM-Fuse-cmlc2-eGFP

Plasmid

#117788

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-1-413aa-YFP

Plasmid

#29599

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-413

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R514G-YFP

Plasmid

#29616

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R514G

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-453aa-YFP

Plasmid

#29600

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-453aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-453

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-mPlum-IFP fusion

Plasmid

#49768

Purpose

For near infrared in vivo optical imaging

Depositor

Yi-Jang Lee

Insert

mPlum-IFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-1-270aa-YFP

Plasmid

#29597

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-270aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-270

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
GST-FUS ΔNLS 1-513

Plasmid

#44984

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS ΔNLS (1-513aa) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 1-513

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R524T-YFP

Plasmid

#29627

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R524T

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R518K-YFP

Plasmid

#29621

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R518K

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
HOXC13(human) GSTfusion-pET

Plasmid

#8680

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R514S-YFP

Plasmid

#29617

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R514S

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
HOXB6 (human) EGFP-fusion

Plasmid

#8535

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains eGFP fused to N-terminal of HOXB6 at AA…

Promoter

CMV

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R524S-YFP

Plasmid

#29626

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R524S

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R522G-YFP

Plasmid

#29625

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R522G

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pUC19_msfGFP-FUS-repair-tempate

Plasmid

#237684

Purpose

For knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Insert

FUS (FUS Human)

Use

CRISPR

Tags

msfGFP

Expression

Mutation

Promoter

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

Plasmid

#222852

Purpose

Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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FUS-9YS-miRFP670-TRF1

Plasmid

#227381

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 9YS (TERF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

9YS

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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FUS-27YS-miRFP670-TRF1

Plasmid

#227383

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 27YS (TERF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

27YS

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS-15YS-miRFP670-TRF1

Plasmid

#227382

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 15YS (TERF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

15YS

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS-5YS-miRFP670-TRF1

Plasmid

#227380

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 5YS (TERF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

5YS

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge