Addgene

We narrowed to 32,186 results for: IND

Showing: 941 - 960 of 32186 results

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Actin-OptoNB

Plasmid

#159595

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

ActinNB with AsLOV2 at loop 5 with 62-666

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MAPT_Halo_C_allele

Plasmid

#178143

Purpose

Donor vector for endogenous tagging of human MAPT at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (MAPT Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Osh1

Plasmid

#58835

Purpose

Expresses GFP-Tagged Osh1 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Osh1 (SWH1, OSH1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1 RagB shRNA #1

Plasmid

#26627

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
LaM8-AK74

Plasmid

#159594

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at AK74

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRset-OppA

Plasmid

#207869

Purpose

Bacterial expression of poly-his-tagged L. lactic OppA

Depositor

Mathew Tantama

Article

Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

Insert

OppA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a D832A

Plasmid

#113517

Purpose

Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

D832A

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-TNKS-2-M1054V

Plasmid

#34692

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2-M1054V (TNKS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

M1054V

Promoter

CMV

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagB shRNA #2

Plasmid

#26628

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
LaM8-GG15-irFP

Plasmid

#159591

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Use

Tags

6 His tag

Expression

Bacterial

Mutation

Promoter

pBAD promotor

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Promoter

Available since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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APP_Halo_C_allele

Plasmid

#178137

Purpose

Donor vector for endogenous tagging of human APP at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (APP Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15

Plasmid

#159593

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

H759A

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FlipSuc-90u

Plasmid

#13558

Depositor

Wolf Frommer

Article

Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

Insert

FlipThuE W283A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W283A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2f

Plasmid

#105065

Purpose

A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2f

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pet11a-ABLE

Plasmid

#158627

Purpose

Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

Depositor

William DeGrado

Article

Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

Insert

Apixaban-Binding Helical Bundle

Use

Tags

6x His-tag and TEV cleavage tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNa-delta S

Plasmid

#107983

Purpose

bacterial expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNa 1080bp-17aa signal (SPP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL-matMYC

Plasmid

#115639

Purpose

For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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