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We narrowed to 25,129 results for: crispr

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Showing: 941 - 960 of 25129 results

  2. pSL690

    Plasmid
    #47753
    Purpose
    Expresses dCas9-VP64 fusion
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47753-plasmid-map-sequence-id-68212

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  3. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Mutation
    D10A nickase
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

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  5. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid
    #112021
    Purpose
    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha
    Depositor
    Alexander Marson
    Publication
    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NYESO beta/alpha into TRAC HDRT
    Use
    CRISPR and Synthetic Biology
    Available since
    Sept. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112021-plasmid-map-sequence-id-215596

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  8. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91248-plasmid-map-sequence-id-192174

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  11. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91249-plasmid-map-sequence-id-181276

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  12. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid
    #112677
    Purpose
    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde
    Insert
    Nuclear-localized floxed-mCherry EGFP
    Use
    AAV
    Tags
    Nuclear localization signal
    Promoter
    EF1a
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112677-plasmid-map-sequence-id-237606

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  14. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62192-plasmid-map-sequence-id-156364

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  15. pMK-Cas9-gate

    Plasmid
    #113743
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.
    Depositor
    Magdalena Bezanilla
    Publication
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR and Gateway Cloning
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113743-plasmid-map-sequence-id-220965

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  20. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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Showing: 941 - 960 of 25129 results