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pRK5-LYCHOS (GPR155) P44A-HA

Plasmid

#199675

Purpose

Transient expression of LYCHOS (GPR155) P44A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P44 mutated to A

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) E48Q-HA

Plasmid

#199662

Purpose

Transient expression of LYCHOS (GPR155) E48Q mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E48 mutated to Q

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) F43I-HA

Plasmid

#199674

Purpose

Transient expression of LYCHOS (GPR155) F43I mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F43 mutated to I

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-DSep1-msfGFP

Plasmid

#174494

Purpose

bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

Sep1 (Sep1 Fly)

Use

Tags

His6-TEV and msfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ubc promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_C93A

Plasmid

#164937

Purpose

Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Expression

Mutation

Promoter

PGK promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

Plasmid

#156417

Purpose

8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

murine Dll4 F2-6/F8 ETS site B/ site #2, 8X

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

E1b min pro/b-globin intron

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-mRFP

Plasmid

#117787

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]

Plasmid

#100962

Purpose

Contains genes encoding GPP synthase, cineole synthase and IspA(S81F)

Depositor

Taek Soon Lee

Insert

GPPS-CSstr-ispA*

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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ELL1 Streptag

Plasmid

#127278

Purpose

Expresses mouse ELL1 with streptactin tag in mammalian cells

Depositor

Christine Milcarek

Insert

ELL1 (NM_007924) (ELL Mouse)

Use

Tags

Twin strep-tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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iGFP-gamma-actin

Plasmid

#231554

Purpose

Mammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

ACTG1 (ACTG1 Human)

Use

Tags

msfGFP (intramolecular)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc sfCherry2ΔC4-Lifeact

Plasmid

#231557

Purpose

Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Lifeact (ABP140 Budding Yeast)

Use

Tags

sfCherry2

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN-PAmCherry-mSNCA-NE

Plasmid

#102364

Purpose

Lentiviral overexpression of PA-mCherry synuclein alpha fusion

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Inserts

PA-mCherry
synuclein alpha (Snca Mouse)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Hsp68

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-MYC-t2A-BCL2

Plasmid

#135306

Purpose

Overexpression of multiple human genes (BCL2 and MYC)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

MYC (MYC Human)
BCL2 (BCL2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Shh

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7

Plasmid

#180315

Purpose

mammalian expression of human SEPT7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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plenti-FLAG-CAMK2D-ED

Plasmid

#221700

Purpose

Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Use

Lentiviral

Tags

Expression

Mutation

K43R, D136N

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-eGFP-SV40

Plasmid

#117811

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Article

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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