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We narrowed to 16,181 results for: nes

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Showing: 941 - 960 of 16181 results

  3. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    60365-plasmid-map-sequence-id-256737

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  4. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    60367-plasmid-map-sequence-id-456413

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  5. nesprin TSmod 25

    Plasmid
    #125555
    Purpose
    Nesprin-2G force sensor with cerulean-F25-venus TSmodule
    Depositor
    Daniel Conway
    Publication
    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mini-nesprin-2G (Syne2 Mouse)
    Tags
    cerulean, venus
    Expression
    Mammalian
    Mutation
    TSmod changed from mTFP to cerulean, flageliform …
    Promoter
    CMV
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125555-plasmid-map-sequence-id-244038

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  6. mCardinal-Farnesyl-5

    Plasmid
    #56159
    Purpose
    Localization: Plasma Membrane, Excitation: 604, Emission: 659
    Depositor
    Michael Davidson
    Publication
    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Farnesyl (HRAS Human)
    Tags
    mCardinal
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  7. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  11. pCAGGS-6011nes

    Plasmid
    #108652
    Purpose
    An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
    Depositor
    Michiyuki Matsuda
    Publication
    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hyBRET-ERK-EV
    Tags
    XFP
    Expression
    Mammalian
    Promoter
    pCAGGS
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    108652-plasmid-map-sequence-id-271043

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  15. pSBbi_RP_MicroID2-FLAG-NES

    Plasmid
    #188525
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188525-plasmid-map-sequence-id-372837

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  17. pcDNA-NES-ZapCY2

    Plasmid
    #59016
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pCMV-dCas13-inactive M3M14nes

    Plasmid
    #157855
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157855-plasmid-map-sequence-id-307628

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  20. PRINCE-SpCas9-1

    Plasmid
    #251235
    Purpose
    PRINCE drug inducible gene editing system based on SpCas9 (Lentiviral-Part II: the SpCas9 expression is under drug-mediated control)
    Depositor
    Yu Wang
    Publication
    Zhang et al Sci Transl Med. 2026 May 27;18(851):eadx
    Insert
    EF1α-SpCas9; 2×NES; 2×ERT2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    July 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251235-plasmid-map-sequence-id-501684

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Showing: 941 - 960 of 16181 results