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pCAG-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171837

Purpose

Mammalian expression of human WIPI2d K128E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

K128E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dH85E-cs-TEV-STREP

Plasmid

#171831

Purpose

Mammalian expression of human WIPI2d H85E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

H85E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dK88E-cs-TEV-STREP

Plasmid

#171832

Purpose

Mammalian expression of human WIPI2d K88E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

K88E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dI92E-cs-TEV-STREP

Plasmid

#171833

Purpose

Mammalian expression of human WIPI2d I92E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

I92E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dC93E-cs-TEV-STREP

Plasmid

#171834

Purpose

Mammalian expression of human WIPI2d C93E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

C93E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2 gRNA resistant

Plasmid

#163408

Purpose

Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

gRNA-targeting sequences are substituted with nuc…

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔEC gRNA resistant

Plasmid

#163413

Purpose

Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The extracellular domain (27-150 aa) is deleted. …

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔTail gRNA resistant

Plasmid

#163412

Purpose

Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The C-terminal tail domain (530-1038 aa) is delet…

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Scarlet-Borcs7-Q87X

Plasmid

#118751

Purpose

Expresses scarlet tagged Borcs7-Q87X

Depositor

Beverly Koller

Insert

Borcs7-Q87X cDNA (2010012O05Rik Mouse)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Q87X

Promoter

CAG

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBW706_pCAG-PV2-lox2272-GFP

Plasmid

#87570

Purpose

PV2 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CD4-YEML-myc

Plasmid

#58538

Purpose

Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Use

Tags

IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…

Expression

Mammalian

Mutation

Added C-terminal Tyrosine-Glutamate-Methionine-Le…

Promoter

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-RFP-miR-E2-4int

Plasmid

#19824

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

miRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-mCherry-FKBP-GEF(ARHGEF1)

Plasmid

#85152

Purpose

acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-roxSTOProx-tRFP-pA-lox

Plasmid

#196888

Purpose

Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

turbo (t)RFP

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-CBE4max-SpRYc-P2A-EGFP

Plasmid

#208336

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CAG

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.2.12.1 Spike

Plasmid

#186032

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.12.1 Spike

Depositor

Marceline Côté

Article

Gong et al Viruses. 2022 Sep 30;14(10):2178. doi: 1

Insert

SARS-CoV-2 BA.2.12.1 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-BE4max-P2A-EGFP (pRZ33)

Plasmid

#140083

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

Depositor

Keith Joung

Insert

Human codon optimized pCAG BE4max with P2A-EGFP

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

D10A (SpCas9)

Promoter

CAG

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-dNS-SRRM1-V5-mCherry

Plasmid

#235095

Purpose

SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)

Use

Tags

Expression

Mammalian

Mutation

The SRRM1 entry clone from DNASU was modified usi…

Promoter

CAG

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-EGFP-WPRE

Plasmid

#51502

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4-5HT3 HC IRES EGFP

Plasmid

#119740

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F, R420Q, R424D, R428A

Promoter

CAG

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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