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We narrowed to 1,740 results for: plasmid for e coli

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Showing: 941 - 960 of 1740 results

  1. pJA12 Mamu-A1*001:01

    Plasmid
    #180442
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*001:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    180442-plasmid-map-sequence-id-355627

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  2. pJA13 Mamu-A1*002:01

    Plasmid
    #180443
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*002:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180443-plasmid-map-sequence-id-355622

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  3. pTCF122 Mamu-B*008:01

    Plasmid
    #180455
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-B*008:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180455-plasmid-map-sequence-id-355081

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  5. pAC-CANTHipi

    Plasmid
    #53301
    Purpose
    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtO
    Use
    Low copy number bacterial cloning vector
    Tags
    6His
    Promoter
    Trc
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53301-plasmid-map-sequence-id-99820

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  7. pAC-aZEA

    Plasmid
    #53286
    Purpose
    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Promoter
    lac
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53286-plasmid-map-sequence-id-83099

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  10. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  11. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  12. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

    Enlarge

  13. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  14. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

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  15. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

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  16. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162684-plasmid-map-sequence-id-321526

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  17. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162670-plasmid-map-sequence-id-321452

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  18. pCJ205

    Plasmid
    #162676
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224A, C529A; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162676-plasmid-map-sequence-id-320828

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  19. pCJ198

    Plasmid
    #162671
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    F495I; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162671-plasmid-map-sequence-id-321447

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  20. pCJ196

    Plasmid
    #162669
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S225A; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162669-plasmid-map-sequence-id-321445

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Showing: 941 - 960 of 1740 results