We narrowed to 21,881 results for: tes

1. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

   Plasmid

   #61582

   Purpose

   Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   Cezanne2 (OTUD7A Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Isoform 2. Deleted aa 463-933.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-CMV-Luc2-IRES-ZsGreen-W

   Plasmid

   #164432

   Purpose

   Lentiviral vector backbone encoding firefly luciferase and ZsGreen

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Inserts

   Luciferase
   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)-ExRai-AKAR2

   Plasmid

   #161753

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-pmExRai-AKAR2

   Plasmid

   #161755

   Purpose

   Plasma membrane-targeted ExRai-AKAR2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   Lyn-ExRai-AKAR2
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-Miro1 S156A

   Plasmid

   #127588

   Purpose

   Phospho-mutant of S156

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S156A

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-mitoExRai-AKAR2

   Plasmid

   #161757

   Purpose

   ExRai-AKAR2 targeted to the outer mitochondrial membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   DAKAP-ExRai-AKAR2
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-erExRai-AKAR2

   Plasmid

   #161756

   Purpose

   ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   CYP450-ExRai-AKAR2
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-Miro1 S156E

   Plasmid

   #127589

   Purpose

   Phospho-mimetic of S156

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S156E

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHDM-Hgpm2

    Plasmid

    #164441

    Purpose

    Encodes codon-optimized HIV gagpol for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    Codon Optimized HIV-1 GagPol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. huCaV2.2 (pSAD442-1)

    Plasmid

    #62574

    Purpose

    Human CaV2.2 expression in mammalian cells

    Depositor

    Diane Lipscombe

    Article

    Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

    Insert

    CACNA1B (CACNA1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRC-CMV-Rev1b

    Plasmid

    #164443

    Purpose

    Encodes HIV-1 Rev accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHDM-VSV-G

    Plasmid

    #164440

    Purpose

    Encodes VSV-G protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    VSV-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-ExRai-AKAR2(T/A)

    Plasmid

    #161754

    Purpose

    Negative-control mutant for ExRai-AKAR2 biosensor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2(T/A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

    Plasmid

    #61590

    Purpose

    Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

    Depositor

    David Komander

    Article

    Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

    Insert

    TRABID (ZRANB1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-244 and aa 698-708.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge