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pLentiCRISPR V2 sgIREB2_1

Plasmid

#169894

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag

Plasmid

#231594

Purpose

Express high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Kozak sequence-Tornado-10xSunTag-2xALFATag

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh20.1 GFP

Plasmid

#209106

Purpose

GFP expressing scramble of shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh20.1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16B

Plasmid

#66607

Purpose

Expresses Sec16B as a GFP fusion

Depositor

David Stephens

Article

Budnik et al Sci Rep. 2011;1:77. doi: 10.1038/srep000

Insert

Sec16B (SEC16B Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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GreenT-EC - GPI

Plasmid

#193946

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Tags

GPI-anchor and Igk_secretion - HA-Flag

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTub-Wg

Plasmid

#64321

Purpose

Expresses Drosophila Wingless protein under the tubulin promotor

Depositor

Roel Nusse

Article

Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8

Insert

Wingless (wg Fly)

Use

Drosophila expression

Promoter

alpha-1 tubulin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBTK1006 (pSL5)

Plasmid

#191001

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBK-CMV Pig10

Plasmid

#16504

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig10 (ENC1 Human)

Expression

Mammalian

Available Since

May 12, 2010

Availability

Academic Institutions and Nonprofits only
pBN1

Plasmid

#86710

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

Insert

Plmn-1::mCherry::his-58::pie-1 3'UTR

Expression

Worm

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSK221

Plasmid

#129249

Purpose

reverse yTRAP, tetR-responsive Neon reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

pADH1(tetO2)-mNeonGreen

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Expression

Insect

Promoter

vasa

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET14b_tev_PSG

Plasmid

#59731

Purpose

Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

TJP1 (TJP1 Human)

Tags

HIS and TEV

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pTriEx-mCherry-Zdk1-TIAM1 DH/PH

Plasmid

#81129

Purpose

mammalian expression of Zdk1 tagged Tiam1 Dh/PH

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
TIAM1 DH/PH (TIAM1 Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIAL1

Plasmid

#106096

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIAL1

Use

CRISPR

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCGN-S2P-WT

Plasmid

#32957

Purpose

For mammalian expression of human S2P with an HA tag

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

S2P (MBTPS2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only
Codon Optimized Dysferlin

Plasmid

#67878

Purpose

The human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.

Depositor

Matthew Hirsch

Article

Hirsch et al Mol Ther. 2013 Dec;21(12):2205-16. doi:

Insert

codon optimized Dysferlin

Use

Unspecified

Tags

No

Promoter

No Promoter

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRRP12_K

Plasmid

#146766

Purpose

Mammalian Expression of HsRRP12

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12 (RRP12 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL358

Plasmid

#169147

Purpose

Encodes OptoQ-AMP1

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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