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pRRL-VinculinI997A_TurboID_Venus

Plasmid

#200273

Purpose

Lentivirus vector expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinI997A_TurboID_Venus

Use

Lentiviral

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG165

Plasmid

#202584

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG161

Plasmid

#202580

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG160

Plasmid

#202579

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-p62 var2

Plasmid

#204576

Purpose

Expresses 3xFLAG-p62 var in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Expression

Mammalian

Mutation

None

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG159

Plasmid

#202578

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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cytoDCIP

Plasmid

#193861

Purpose

Encodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry-GFP1-10

Expression

Plant

Promoter

35S

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI-1P

Plasmid

#202618

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Expression

Insect

Promoter

hsp70

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLT237

Plasmid

#174300

Purpose

Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

PolC homology arm

Expression

Bacterial

Mutation

changed Cysteine 669 to Tyrosine

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

Plasmid

#199454

Purpose

U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS2 sgRNA

Use

CRISPR

Promoter

mouse U6

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA28-pMa-PspCas13b crRNA-TS1

Plasmid

#199459

Purpose

U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS1 crRNA

Use

CRISPR

Promoter

mouse U6

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

Plasmid

#199460

Purpose

Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

Zebrafish NarTag

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_57-684_J

Plasmid

#146694

Purpose

Insect Expression of DmHPat_57-684

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-684 (Patr-1 Fly)

Expression

Insect

Mutation

Two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-del57-499_H

Plasmid

#146460

Purpose

Insect Expression of DmHPat-del57-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499 (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation C2340A compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_1-499_H

Plasmid

#146461

Purpose

Insect Expression of DmHPat_1-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_1-499 (Patr-1 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_1-684_H

Plasmid

#146462

Purpose

Insect Expression of DmHPat_1-684

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_1-684 (Patr-1 Fly)

Expression

Insect

Mutation

Two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_57-499_H

Plasmid

#146463

Purpose

Insect Expression of DmHPat_57-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-499 (Patr-1 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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