We narrowed to 7,571 results for: Lif;

1. pBSR (mCherry-surrogateTarget-bsr)

   Plasmid

   #66950

   Purpose

   Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   blasticidin
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPO635

   Plasmid

   #195297

   Purpose

   pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABC operon of McCAST (Tn7575)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   IPTG inducible lac promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW300ParsR-Amp32T

   Plasmid

   #78652

   Purpose

   pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pMH-HT_ygiF-CHAD

   Plasmid

   #127793

   Purpose

   Expresses the CHAD domain of ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF-CHAD (ygiF Escherichia coli)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   N-terminal TTM domain of ygiF (residues 201-433)

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH131

   Plasmid

   #48260

   Purpose

   Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

   Inserts

   URA3 3' fragment
   URA3 5' fragment
   GAL10/GAL1 bidirectional promoter
   

   Use

   Routine cloning vector

   Tags

   Expression

   Mutation

   -242 upstream to URA3 ORF +495 and URA3 ORF from …

   Promoter

   GAL10/GAL1 bidirectional

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-3xflagGREB1b

   Plasmid

   #112222

   Purpose

   mammalian expression of flag tagged GREB1b

   Depositor

   Craig Burd

   Article

   Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

   Insert

   GREB1b (GREB1 Human)
   

   Use

   Tags

   3x flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIhh742bp-Luc

   Plasmid

   #53605

   Purpose

   Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

   Depositor

   Xiangli Yang

   Article

   Wang et al Development. 2009 Dec;136(24):4143-53. d

   Insert

   Ihh promoter
   

   Use

   Luciferase; Reporter

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pOPO636

   Plasmid

   #195299

   Purpose

   pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABCQ operon of McCAST (Tn7575)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   IPTG inducible lac promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. NET33 pmRFP-N2 (448)

    Plasmid

    #61979

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET33 (SCARA5 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only