We narrowed to 17,163 results for: EMB

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Tags

HA

Expression

Mammalian

Mutation

E186Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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POLArISact

Plasmid

#164971

Purpose

Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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TUBG1

Plasmid

#87954

Purpose

expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

whole protein, The C-terminal is a sh-gamma-tubul…

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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HUWE1-sgRNA-2

Plasmid

#86925

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA2 (HUWE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only
pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pRDA_889

Plasmid

#216080

Purpose

ITRs from AAV2, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

ITRs from AAV2

Use

AAV; Destination

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-pontin

Plasmid

#53608

Purpose

Retroviral expression of human pontin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

pontin (RUVBL1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

pontin CDS mutated to confer resistance to shRNA.…

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pCAX-mScarlet-CIP4

Plasmid

#178364

Purpose

Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGM8

Plasmid

#220166

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TSPO (TSPO Human)

Use

Lentiviral

Tags

GFP11

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(WT)

Plasmid

#206406

Purpose

Mammalian expression of hARL1(WT)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-SEC61B-mCherry-FRB

Plasmid

#233349

Purpose

Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

SEC61B (SEC61B Human)
mCherry
FRB

Use

Retroviral

Expression

Mammalian

Mutation

T2098L mutant of FKBP-rapamycin binding domain of…

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-DAXX-mSIM1/2

Plasmid

#134525

Purpose

Expresses YFP-DAXX fusion protein with a mutations in both its 5' and 3' SIMs

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1/2

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG and SIM (IIVL)…

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-hDF C2B

Plasmid

#72583

Purpose

human dysferlin C2B domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2B domain (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

C2B domain

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pBRPyCAG-hKlf2-DsRed-Ip

Plasmid

#26277

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Klf2 (KLF2 Human)

Expression

Mammalian

Available Since

Nov. 12, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-LIR-mutant (LIRAAAA)

Plasmid

#187981

Purpose

Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DDDW335-338AAAA (LIR mutant), silent mutation siR…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1

Plasmid

#208862

Purpose

Stably express GFP-tagged NAP1 for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-EYFP-P2A-Mafb

Plasmid

#98175

Purpose

Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Mafb

Depositor

Oscar Marin

Article

Mi et al Science. 2018 Feb 22. pii: science.aar68

Insert

Mafb (Mafb Mouse)

Use

Retroviral

Promoter

CAG

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL-gRNA(anti-cGAS)-Cas9-Puro

Plasmid

#130908

Purpose

gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Cas9

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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