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We narrowed to 30,593 results for: TRO

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Showing: 9681 - 9700 of 30593 results

  5. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  6. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid
    #140004
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140004-plasmid-map-sequence-id-275398

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  7. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

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  12. pMG

    Plasmid
    #58991
    Purpose
    MSCV based expression vector - expresses GFP
    Depositor
    David Baltimore
    Article
    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:
    Type
    Empty backbone
    Use
    Retroviral; Mscv-based retroviral system that exp…
    Expression
    Mammalian
    Promoter
    MSCV
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pUB2.1

    Plasmid
    #40728
    Purpose
    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
    Depositor
    Thomas Hughes
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40728-plasmid-map-sequence-id-128586

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  14. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-489173

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  15. pcDNA3.1-KozATG-dL5-2XG4S-actin

    Plasmid
    #73262
    Purpose
    Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.
    Insert
    KozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)
    Tags
    The FAP and actin are fused with 2 copies of a G4…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73262-plasmid-map-sequence-id-137068

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  20. pQCXIP-Laforin Myc

    Plasmid
    #84656
    Purpose
    mammalian expression and/or retrovirus preparation
    Depositor
    Ayano Satoh
    Article
    Toyota et al Sciencematters
    Insert
    Laforin (EPM2A Human)
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    5'LTR
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 9681 - 9700 of 30593 results