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pSens-4OmeNB

Plasmid

#216231

Purpose

Expresses GFP in response to 4'-O-Methylnorbelladine

Depositor

Simon d'Oelsnitz, Andrew Ellington

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Inserts

RamR-4NB2
sfGFP

Use

Synthetic Biology

Mutation

K64T, L67M, C135D, S138G

Promoter

J23103 and Pramr

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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EasyFusion Halo-mAID

Plasmid

#112852

Purpose

General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Halo-mAID

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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Stx3

Plasmid

#186685

Purpose

Expresses Syntaxin 3 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Syntaxin 3

Tags

6 his tag

Expression

Bacterial

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-miR-9 sponge

Plasmid

#25040

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 sponge

Use

Retroviral

Tags

d2EGFP

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pemiRFP703-clathrin

Plasmid

#136563

Purpose

Clathrin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703-clathrin

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_right

Plasmid

#187846

Purpose

expresses the right-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_left

Plasmid

#187845

Purpose

expresses the left-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBMN-FKBP DD(L106P)-UbK0G75S-sfGFP

Plasmid

#170157

Purpose

Expression for LIBRON constructs

Depositor

Thomas Wandless

Article

Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

Insert

FKBP DD (L106P)-UbK0G75S

Use

Retroviral

Tags

sfGFP

Mutation

change all lysin residues of ubiquitin to Arg, G7…

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-mCherry

Plasmid

#79509

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

mCherry RFP

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pNup133-Ypet

Plasmid

#98283

Purpose

mammalian expression of Nup133-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup133 (NUP133 Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Zeo-CnB

Plasmid

#191965

Purpose

Expression of CnB (calcineurin subunit beta).

Depositor

Luca Scorrano

Article

Cereghetti et al Proc Natl Acad Sci U S A. 2008 Oct 14;10

Insert

Calcineurin subunit beta (PPP3CB Human)

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CRY2low-mCherry

Plasmid

#104065

Purpose

Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2low-mCherry

Expression

Mammalian

Mutation

CRY2 aa 1-491 truncation, R489E A491D

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR R4-vas2-integrase-R3

Plasmid

#62299

Purpose

phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

phageC31 integrase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pmiRFP720-tubulin

Plasmid

#136574

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP720

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP720-tubulin

Tags

miRFP720

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCoofy44

Plasmid

#55185

Purpose

Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

Expression

Bacterial

Promoter

pPH (Polyhedrin promoter)

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCfB6633

Plasmid

#106162

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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FusionRed-Lifeact-7

Plasmid

#54778

Purpose

Localization: Actin, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Lifeact-FusionRed

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLP16_Lenti Scramble Control

Plasmid

#239417

Purpose

Negative control Lentiviral plasmid for SpCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble sequence

Use

Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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B23(9-122)

Plasmid

#23142

Depositor

SeWon Suh

Article

Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

Insert

B23 core (NPM1 Human)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-8

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only