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pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCambia-dLB-kanR-GFP

Plasmid

#223177

Purpose

Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

Depositor

Andreas Stuermer

Type

Empty backbone

Use

Ti plasmid for agrobacterium

Expression

Plant

Available since

Aug. 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.E2.ChETA

Plasmid

#220310

Purpose

Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

Depositor

Matt Rowan

Publication

Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

Insert

ChETA

Use

AAV

Expression

Mammalian

Promoter

S5E2

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(HA)

Plasmid

#195022

Purpose

Gateway vector containing HA-tagged Creb5

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Gateway Cloning

Tags

3xHA

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

Plasmid

#211906

Purpose

Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

Depositor

Yannick Doyon

Publication

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD22-BBz

Use

AAV

Expression

Mammalian

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mStrawberry-15b

Plasmid

#206868

Purpose

Express tTALE-mStrawberry

Depositor

Kyubong Jo

Publication

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mStrawberry

Expression

Bacterial

Promoter

T7 promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo4

Plasmid

#203925

Purpose

Cloning plasmid containing the R6Kγ origin and oriT insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

R6Kγ origin of replication and oriT

Expression

Plant

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p5204

Plasmid

#168181

Purpose

bacteria reporter

Depositor

Michael Hensel

Publication

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PcypD::sfgfp (frameshift)

Expression

Bacterial

Mutation

frameshift sfGFP

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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p5055

Plasmid

#168178

Purpose

bacteria reporter

Depositor

Michael Hensel

Publication

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PhtrA::sfgfp

Expression

Bacterial

Mutation

none

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1

Plasmid

#197395

Purpose

Expresses SUMO-tagged mouse METAP1

Depositor

David Giedroc

Publication

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 (Metap1 Mouse)

Tags

SUMO

Expression

Bacterial

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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p5084

Plasmid

#168179

Purpose

bacteria reporter

Depositor

Michael Hensel

Publication

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PmsrA::sfgfp

Expression

Bacterial

Mutation

none

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-Y207F+W275L

Plasmid

#196022

Purpose

Expresses CRKL Y207F + W275L

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-CDS1-3HA

Plasmid

#184760

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

CDS1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

DGK1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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ABF2-2EGFP-ter-URA3

Plasmid

#184768

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ABF2 (ABF2 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_StefinA

Plasmid

#182402

Purpose

Bacterial expression of stefinA

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

stefin (CSTA Human)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMAT36

Plasmid

#192979

Purpose

BHR plasmid (pRA301) expressing mirA-3XFLAG from T7 promoter for high yield protein purification

Depositor

Clay Fuqua

Publication

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

mirA-3xFLAG

Tags

3XFLAG

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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VAMP8

Plasmid

#186689

Purpose

Expresses VAMP8 in E. Coli

Depositor

Axel Brunger

Publication

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

VAMP8

Tags

3xFLAG, GST, TEV, and Thrombin

Expression

Bacterial

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-trAtCCD8(pYL736)

Plasmid

#178287

Purpose

Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pZCAG

Plasmid

#129114

Purpose

Z. rouxii centromeric vector containing G418 resistance

Depositor

Paolo Giudici

Publication

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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