163,421 results

1. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDESTmycYBX1

   Plasmid

   #19878

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Y box binding protein 1 (YBX1 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-L274GMmMetRS-T2A-mCherry

   Plasmid

   #220800

   Purpose

   Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

   Depositor

   Elizabeth Kirby

   Article

   Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

   Insert

   Mars1 (Mars1 Mouse)
   

   Use

   Lentiviral

   Tags

   2xFLAG and T2A-mCherry

   Expression

   Mutation

   mutation in MetRS to change aa 274 from L to G

   Promoter

   EF1a

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1A-CD19.28H.28TM.28.3z

   Plasmid

   #200678

   Purpose

   Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOsTIR1w/oGFP

   Plasmid

   #102883

   Purpose

   Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   OsTIR1
   

   Use

   Tags

   3x myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Puro-sgNT

   Plasmid

   #138681

   Purpose

   Expresses a non-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNT
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ChR2-2A-Halo

   Plasmid

   #22047

   Purpose

   Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

   Depositor

   Edward Boyden

   Article

   Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

   Insert

   ChR2-GFP-2A-Halo-YFP
   

   Use

   Tags

   GFP and YFP

   Expression

   Mammalian

   Mutation

   Note that there is a S147R mut in YFP. This mutat…

   Promoter

   CMV

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

   Plasmid

   #176285

   Purpose

   Cre-dependent AAV virus

   Depositor

   The Allen Institute for Brain Science, Shenqin Yao

   Article

   Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

   Insert

   TVA66T-dTomato-rabies Glycoprotein
   

   Use

   Tags

   dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FASTHDR-Cterm-mClover3-Puromycin

   Plasmid

   #167205

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L

    Plasmid

    #196136

    Purpose

    SB vector for the mammalian expression of GADD34-K3L

    Depositor

    Jamie Cate

    Article

    Aleksashin et al bioRxiv. 2023 Feb 10:2023.02.09.527910.

    Insert

    GADD34 Δ1-240 - P2A - K3L (PPP1R15A Human, vaccinia virus)
    

    Use

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ131C

    Plasmid

    #69284

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. SARS-CoV2 Nucleocapsid

    Plasmid

    #153201

    Purpose

    Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

    Depositor

    Peter Klein

    Article

    Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

    Insert

    Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    

    Use

    Tags

    Myc tag (5X)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCDH1-CMV-HT-LC3-SV40-Hygro

    Plasmid

    #182045

    Purpose

    Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

    Depositor

    Hong-Gang Wang

    Article

    Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    HaloTag-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDawn-AmpR-MCS

    Plasmid

    #188978

    Purpose

    For red light-induced bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Rictor_1 shRNA

    Plasmid

    #1853

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLU-YFP-ICP0

    Plasmid

    #134563

    Purpose

    Expresses YFP-ICP0 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

    Insert

    YFP-ICP0
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ132C

    Plasmid

    #69285

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFB2E

    Plasmid

    #195288

    Purpose

    Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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