We narrowed to 22,513 results for: scr

1. pYPQAT40

   Plasmid

   #223412

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROP_C9_Puro_TOP1

   Plasmid

   #183323

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   TOP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGADT7-αC

   Plasmid

   #197650

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-2 αC
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Ser677T substitution

   Promoter

   ADH1

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROP_C9_Puro_AAVS1A

   Plasmid

   #183337

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   AAVS1A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP_C9_Puro_AAVS1B

   Plasmid

   #183338

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   AAVS1B
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROP_C9_Puro_TOP2B

   Plasmid

   #183325

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   TOP2B
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROP_C9_Puro_TOP2A

   Plasmid

   #183324

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   TOP2A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmIL-6 FL

   Plasmid

   #61286

   Purpose

   drives luciferase from Wt mouse IL-6 promoter (full length)

   Depositor

   Gail Bishop

   Article

   Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

   Insert

   IL-6 promoter (Il6 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   IL-6 full length promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMLS-SV40-EGFP

   Plasmid

   #46919

   Purpose

   Target EGFP gene that is stably integrated into HEK293 genome

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   GFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-NRF1-HA

    Plasmid

    #34708

    Depositor

    Raymond Deshaies

    Article

    Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

    Insert

    NRF1 (NFE2L1 Human)
    

    Use

    Retroviral

    Tags

    3XFlag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. BirA-Myc_N_PAX6

    Plasmid

    #167721

    Purpose

    Expresses PAX6 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    PAX6 (PAX6 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV BAF170-Flag

    Plasmid

    #19142

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-NRF1

    Plasmid

    #34707

    Depositor

    Raymond Deshaies

    Article

    Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

    Insert

    NRF1 (NFE2L1 Human)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pPB-PGK-destination

    Plasmid

    #60436

    Purpose

    Destination vector with PGK - Hygromycin selection marker

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP-HA-DAT

    Plasmid

    #90244

    Purpose

    Expresses YFP-HA-DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    residues 193–203 of human DAT were replaced in YF…

    Promoter

    CMV

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE TRE dCas9

    Plasmid

    #50915

    Purpose

    Tet-regulatable dCas9 lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK-EGFP-FKBPF36V

    Plasmid

    #199765

    Purpose

    plasmid for targeting dTAG into the AAVS1 locus

    Depositor

    Hannes Lans

    Article

    van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

    Insert

    GFP-dTAG
    

    Use

    Tags

    GFP and dTAG

    Expression

    Mammalian and Worm

    Mutation

    Promoter

    hPGK

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TadCBE-Cas9NG

    Plasmid

    #225093

    Purpose

    Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

    Insert

    TadCBE-Cas9NG
    

    Use

    CRISPR

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRX-IZ-ANXA7-mRuby3

    Plasmid

    #184893

    Purpose

    Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA7 (ANXA7 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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