We narrowed to 88,746 results for: Mal

1. pAT15546_nABE8e-SuperFi-Cas9

   Plasmid

   #184376

   Purpose

   Mammalian expression of SuperFi-Cas9 ABE8e base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nABE8e-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Frt-hspB9

   Plasmid

   #63100

   Purpose

   Expression of HSPB9 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb9 (HSPB9 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Promoter

   EF1a and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM612 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 in TUPV3

   Plasmid

   #161571

   Purpose

   Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-shRBFOX1-3261

   Plasmid

   #115458

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shRBFOX1-3261 (RBFOX1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-shRBFOX1-3260

   Plasmid

   #115457

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shRBFOX1-3260 (RBFOX1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mouse GFP-PRMT7 Wild Type

   Plasmid

   #65056

   Purpose

   Expresses wild type mouse GFP-PRMT7

   Depositor

   Steven Clarke

   Article

   Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

   Insert

   PRMT7 (Prmt7 Mouse)
   

   Use

   Bacterial propagation

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pF714-pET28a-Hs-FL-METTL20-NHis wt

   Plasmid

   #85115

   Purpose

   expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

9. nDHFR173-gs10-NLS-VPR

   Plasmid

   #188262

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nDHFR173 (DHFR Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDual-eGFP(H66)

    Plasmid

    #63558

    Purpose

    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(H66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCI-neo-myc-LC3(deltaC22)

    Plasmid

    #45448

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Encodes amino acids 1-120

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

13. MDH1-miR-31SM-PGK-GFP-Blast

    Plasmid

    #31516

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-31 (Mir31 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

14. JPF0535a

    Plasmid

    #124042

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB2AR-miRFP670nano

    Plasmid

    #127442

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    B2AR-miRFP670nano (ADRB2 Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPRR2A-pFastBac I

    Plasmid

    #182460

    Purpose

    Expression His-tagged SPRR2A in insect cells

    Depositor

    Lora Hooper

    Article

    Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

    Insert

    small proline rich protein 2A (SPRR2A Human)
    

    Tags

    6xHis

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330 NC-FKBP-Cas9

    Plasmid

    #162725

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Frt-hspB6

    Plasmid

    #63097

    Purpose

    Expression of HSPB6 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb6 (HSPB6 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

    Plasmid

    #108814

    Purpose

    Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    TP901 integrase fragment aa327 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rtn4a-NTD1-135-mNeon

    Plasmid

    #186609

    Purpose

    Encodes Reticulon 4 N-terminal domain aa 1 to 135 fused to mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Human Reticulon 4 isoform A amino acids 1 to 135 (RTN4 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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