We narrowed to 32,462 results for: fer
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Plasmid#191682PurposeDonor plasmid backbone PGK-puroRDepositorTypeEmpty backboneExpressionMammalianMutationWTAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only
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pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR
Plasmid#189943PurposeLentiviral vector to co-express an spsgRNA with NLS-tagBFPDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-R4-bpFOG-L5
Plasmid#186369PurposeEntry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequencesDepositorInsertBasal promoter of the Friend Of Gata gene
UseProtein expressionPromoterT7 promoterAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-pFOGl-L5
Plasmid#186364PurposeEntry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertFriend Of Gata regulatory sequence
UseProtein expressionPromoterT7 promoterAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA
Plasmid#186407PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA
Plasmid#186397PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA
Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-a-element-L4
Plasmid#186366PurposeEntry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequencesDepositorInserta-element early Otx neural enhancer
UseProtein expressionPromoterT7 promoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Venus-stop-L2
Plasmid#186360PurposeEntry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequencesDepositorInsertVenus yellow fluorescent protein
UseExpression of the venus fluorescent proteinAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODALvar-matMYC
Plasmid#115641PurposeFor targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycyclineDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-MYC-DYK-N72A-N199A
Plasmid#115252PurposeFor mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tagDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-MYC-DYK-C312A
Plasmid#115253PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tagDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK-C302S
Plasmid#115254PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tagDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK-C302A
Plasmid#115255PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tagDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK-N328A
Plasmid#115246PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tagDepositorInsertNODAL splice variant (NODAL Human)
TagsMYC-DYKExpressionMammalianMutationN328APromoterCMVAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-HA
Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSub-Asp6-MBP-HRas
Plasmid#185758PurposeBacterial expression of Asp6-MBP-HRasDepositorInsertAsp6-MBP-HRas
TagsMBPExpressionBacterialMutation6x Asp added to the N terminus of MBPPromotertacAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-foxd4l1.1-F>E
Plasmid#185508PurposeExpression of Xenopus laevis foxd4DepositorInsertfoxd4l1.1
UseIn vitro transcriptionMutationF>EAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-foxd4l1.1-A6
Plasmid#185506PurposeExpression of Xenopus laevis foxd4DepositorInsertfoxd4l1.1
UseIn vitro transcriptionMutationFSIENIM to AAAAAAMAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
shRNA-Ratio-NegCon
Plasmid#183554PurposeDesigned to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)DepositorInsertLuciferase shRNA
ExpressionMammalianPromoterCBhAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only