We narrowed to 11,805 results for: nsf

1. FUW-[EF1α-HER1 CD28Z]

   Plasmid

   #218339

   Purpose

   Lentiviral transfer plasmids for mammalian expression HER1 CAR

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   HER1 CAR mammalian expression cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRLSIN-[EF1α-HER1 4-1BB]

   Plasmid

   #218341

   Purpose

   Lentiviral transfer plasmids for mammalian expression HER1 CAR

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   HER1 CAR mammalian expression cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-ASAP5-WPRE

   Plasmid

   #225709

   Purpose

   Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Insert

   ASAP5
   

   Use

   AAV

   Promoter

   human synapsin

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-ASAP5-Kv2.1-WPRE

   Plasmid

   #225707

   Purpose

   Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Has Service

   AAV1

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Promoter

   human synapsin

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-DIO-ATLASsnFlp

   Plasmid

   #232352

   Purpose

   Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Has Service

   AAV8

   Insert

   ATLASsnFlp
   

   Use

   AAV

   Tags

   ALFA-Tag

   Promoter

   hSyn

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-UBC-XRI-HA

   Plasmid

   #178056

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-HA
   

   Use

   AAV

   Tags

   HA-MBP

   Expression

   Mammalian

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GRAB-gDA3m

   Plasmid

   #208698

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Has Service

   AAV1

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

   Plasmid

   #168305

   Purpose

   mediated knock-in of sgRNA precursor

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   SaKKH-EGFP-U6-sgRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1alpha, CBh

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GRAB_ACh3.0

    Plasmid

    #121922

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DIO-BACE-HA

    Plasmid

    #232353

    Purpose

    Beta-Secretase to be expressed (1:1) with either ATLAS protein.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Has Service

    AAV8

    Insert

    BACE (BACE1 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHIV-EGFP.Flag-EZH2

    Plasmid

    #220243

    Purpose

    Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61593

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    HA, NLS, and OLLAS tag

    Expression

    Mammalian

    Mutation

    K175R and K736R (deUb mutations)

    Available Since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CR029

    Plasmid

    #234041

    Purpose

    expresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmid

    Depositor

    Alexander Marson

    Article

    Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

    Insert

    evoCDA1-Cas9(D10A)-P2A-blast
    

    Use

    Lentiviral

    Tags

    blast

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CR102

    Plasmid

    #234043

    Purpose

    expresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmid

    Depositor

    Alexander Marson

    Article

    Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

    Insert

    TadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast
    

    Use

    Lentiviral

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-GRAB-gDA3m

    Plasmid

    #208707

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-GRAB-rDA3m

    Plasmid

    #208708

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-sgR26

    Plasmid

    #127376

    Purpose

    Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-Best1-sCD200

    Plasmid

    #164424

    Purpose

    AAV plasmid expressing soluble CD200 in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    CD200 (Cd200 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    deleted terminal 40 amino acids

    Promoter

    Best1

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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