We narrowed to 5,906 results for: pcDNA 3.1

1. pcDNA3.1-nAChr-Alpha6

   Plasmid

   #48812

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Beta2-PAmcherry1

   Plasmid

   #114190

   Purpose

   studying clustering of Beta2 receptors by superresolution microscopy (PALM)

   Depositor

   Aleksandra Radenovic

   Insert

   Adrenergic receptor beta 2 (ADRB2 Human)
   

   Use

   Tags

   3HA, FLAG, and PAmcherry1

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY019 (pcDNA3.1-hPmCpf1)

   Plasmid

   #69991

   Purpose

   Expresses humanized PmCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hPmCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3.1-hMcl-1(T163A)

   Plasmid

   #25376

   Depositor

   Roger Davis

   Article

   Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

   Insert

   hMCL1 (MCL1 Human)
   

   Use

   Tags

   V5 and His tag

   Expression

   Mammalian

   Mutation

   changed threonine 163 to alanine

   Promoter

   Available since

   Aug. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pY009 (pcDNA3.1-hSsCpf1)

   Plasmid

   #69981

   Purpose

   Expresses humanized SsCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hSsCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1(-)-Green Falcan0.3

   Plasmid

   #199704

   Purpose

   green fluorescent indicator for cAMP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

   Insert

   Green Falcan0.3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(-)-Green Falcan10

   Plasmid

   #199707

   Purpose

   green fluorescent indicator for cAMP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

   Insert

   Green Falcan10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-deltaA1-VWF

   Plasmid

   #124823

   Purpose

   Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF without A1 domain (VWF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted A1 domain

   Promoter

   CMV

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)_Granulin 2-no linker

   Plasmid

   #176921

   Purpose

   express Granulin 2 without linker 3 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 2 (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)_Granulin 1+linker2

    Plasmid

    #176918

    Purpose

    express Granulin 1 with linker 2 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 1+linker2 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1/GS-DJ1-L166P-V5

    Plasmid

    #29343

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His6 and V5 epitope

    Expression

    Mammalian

    Mutation

    L166P

    Promoter

    Available since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+) eGFP PPP1R8 G4

    Plasmid

    #129021

    Purpose

    Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+) eGFP EML2 G4

    Plasmid

    #129023

    Purpose

    Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY015 (pcDNA3.1-hLiCpf1)

    Plasmid

    #69987

    Purpose

    Expresses humanized LiCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLiCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Lifeact-rsLOV2 pcDNA3.1(+)

    Plasmid

    #107882

    Purpose

    Mammalian expression of Lifeact-rsLOV2

    Depositor

    Stefan Hell

    Article

    Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

    Insert

    rsLOV2
    

    Use

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(-)-Green Falcan3

    Plasmid

    #199706

    Purpose

    green fluorescent indicator for cAMP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

    Insert

    Green Falcan3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Ctdnep1_D67E-2xFlag

    Plasmid

    #196519

    Purpose

    Expression of Ctdnep1_D67E-2xFlag

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead)

    Promoter

    CMV promoter; T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

    Plasmid

    #196518

    Purpose

    Expression of Ctdnep1_D67E-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67EsiR (CTDNEP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead). Silent mutatio…

    Promoter

    CMV promoter; T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1+CeGFP-Ctdnep1WTsiR

    Plasmid

    #196517

    Purpose

    Expression of Ctdnep1WT-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1WTsiR (CTDNEP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    CMV promoter; T7 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1/HisC 14-3-3 sigma

    Plasmid

    #11947

    Depositor

    Michael Yaffe

    Article

    Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

    Insert

    Stratifin (SFN Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only