We narrowed to 7,456 results for: Lif

1. Pb mD3S4-dN

   Plasmid

   #180252

   Purpose

   P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

   Depositor

   Timothy Springer

   Article

   Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

   Insert

   D3 of P. berghei HAP2 with N-glycosylation sites mutated
   

   Use

   Tags

   6His

   Expression

   Mammalian

   Mutation

   N516T, S533N and N539Q

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rp51a_10bp 5'SS_No BP or pBP_pUC18

   Plasmid

   #100989

   Purpose

   Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

   Plasmid

   #100990

   Purpose

   Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-eGFP-TAX1BP1 Y635A

   Plasmid

   #175772

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Y635A

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE-eGFP-TAX1BP1 N637A

   Plasmid

   #175773

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   N637A

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NmMetNI-WT

   Plasmid

   #172114

   Purpose

   Expression of MetN and MetI membrane proteins

   Depositor

   Douglas Rees

   Article

   Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

   Inserts

   MetN
   MetI
   

   Use

   Tags

   10x His Tag and Enterokinase

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-WIPI2dR108E-cs-TEV-STREP

   Plasmid

   #171835

   Purpose

   Mammalian expression of human WIPI2d R108E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   R108E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-WIPI2dR125E-cs-TEV-STREP

   Plasmid

   #171836

   Purpose

   Mammalian expression of human WIPI2d R125E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   R125E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-WIPI2dK128E-cs-TEV-STREP

   Plasmid

   #171837

   Purpose

   Mammalian expression of human WIPI2d K128E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   K128E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-WIPI2dH85E-cs-TEV-STREP

    Plasmid

    #171831

    Purpose

    Mammalian expression of human WIPI2d H85E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    H85E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-WIPI2dK88E-cs-TEV-STREP

    Plasmid

    #171832

    Purpose

    Mammalian expression of human WIPI2d K88E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    K88E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-WIPI2dI92E-cs-TEV-STREP

    Plasmid

    #171833

    Purpose

    Mammalian expression of human WIPI2d I92E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    I92E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-WIPI2dC93E-cs-TEV-STREP

    Plasmid

    #171834

    Purpose

    Mammalian expression of human WIPI2d C93E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    C93E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Halo-WIPI2B S395E

    Plasmid

    #176006

    Purpose

    Expresses WIPI2B (S395E) fused to Halo in mammalian cells

    Depositor

    Erika Holzbaur

    Article

    Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

    Insert

    WIPI2B (S395E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Serine 395 to Glutamate

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Halo-WIPI2B S395A

    Plasmid

    #176005

    Purpose

    Expresses WIPI2B (S395A) fused to Halo in mammalian cells

    Depositor

    Erika Holzbaur

    Article

    Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

    Insert

    WIPI2B (S395A) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Serine 395 to Alanine

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. acta1-mRuby2-VHH[GFP]

    Plasmid

    #163856

    Purpose

    Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    VHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT3TS-CD44b-TurboID

    Plasmid

    #163850

    Purpose

    In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    CD44b (cd44b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLIC-lyn11-myc-LOV2GIVe (L)

    Plasmid

    #170624

    Purpose

    For the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539E

    Depositor

    Mikel Garcia-Marcos

    Article

    Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

    Insert

    Lyn11-myc-LOV2GIVe (L)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiCRISPRv2bleo-Nectin-2

    Plasmid

    #170295

    Purpose

    A knock-out vector for the mouse Nectin-2

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse Nectin-2 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LentiCRISPRv2bleo-Necl-5

    Plasmid

    #170294

    Purpose

    A knock-out vector for the mouse Necl-5

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse Necl-5 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLIC-lyn11-myc-LOV2GIVe (D)

    Plasmid

    #170625

    Purpose

    For the mammalian expression of mLOV2GIVe dark-mimicking mutation C450S

    Depositor

    Mikel Garcia-Marcos

    Article

    Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

    Insert

    Lyn11-myc-LOV2GIVe (D)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-TK-Firefly-cjCD69

    Plasmid

    #122279

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjCD69 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-TK-Firefly-cjMGA

    Plasmid

    #122280

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjMGA 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-TK-Firefly-cjPACS1

    Plasmid

    #122281

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjPACS1 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-TK-Firefly-cjSTK4

    Plasmid

    #122282

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjSTK4 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-TK-Firefly-cjTP53RK

    Plasmid

    #122283

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjTP53RK 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-TK-Firefly-cjYTHDC1

    Plasmid

    #122284

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjYTHDC1 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Dcp2C Δ5H-H1

    Plasmid

    #163584

    Purpose

    express Dcp2 FL-H1 at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of aa1-326; the first HLM (EDQLKSYAEEQ…

    Promoter

    DCP2 promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge