We narrowed to 15,742 results for: grna

1. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB9336 (pgRNA_II-1_NatMX)

   Plasmid

   #161589

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CSNK1A1 gRNA (BRDN0001145680)

   Plasmid

   #76189

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PIKFYVE gRNA (BRDN0001147303)

   Plasmid

   #76892

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIKFYVE (PIKFYVE Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PRKDC gRNA (BRDN0001149021)

   Plasmid

   #77864

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKDC

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKDC (PRKDC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-HypaCas9-ECT2_sgRNA

   Plasmid

   #183873

   Purpose

   pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCriprV2-sgRNA-PER1-#1

   Plasmid

   #189987

   Purpose

   Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

   Depositor

   Achim Kramer

   Article

   Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   Period1 (PER1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD2 gRNA (BRDN0001146864)

   Plasmid

   #75827

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CD2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CD2 (CD2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRKDC gRNA (BRDN0001149438)

   Plasmid

   #77863

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKDC

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKDC (PRKDC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid

    #172828

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. STK4 gRNA (BRDN0001147987)

    Plasmid

    #76075

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK4 (STK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-U6-pegRNA_SRD5A3-EGFP

    Plasmid

    #159781

    Purpose

    S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGK1 gRNA (BRDN0001146969)

    Plasmid

    #77982

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PGK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PGK1 (PGK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330-Elf5 gRNA

    Plasmid

    #128836

    Purpose

    gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    mElf5 gRNA (Elf5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CHEK1 C1.2 gRNA

    Plasmid

    #90626

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CHEK1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CHEK1 (Guide Designation C1.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PPP2R2B B6.5 gRNA

    Plasmid

    #90848

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PPP2R2B

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PPP2R2B (Guide Designation B6.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFR gRNA (BRDN0001146570)

    Plasmid

    #75688

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EGFR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSIN-Skil-gRNA2

    Plasmid

    #180371

    Purpose

    targeting mouse Skil/SnoN gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Skil targeting gRNA (Skil Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TRIM28 gRNA (BRDN0001162440)

    Plasmid

    #76140

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Spy EMX1-pegRNA

    Plasmid

    #169855

    Purpose

    SpyCas9-pegRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    Spy EMX1-pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BRD2 gRNA (BRDN0001487068)

    Plasmid

    #77983

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRD2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    BRD2 (BRD2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid

    #64159

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ATM N-terminal sgRNA

    Plasmid

    #207089

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATCATTAAGTACTAGACTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PFKFB3 gRNA (BRDN0001147151)

    Plasmid

    #76461

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PFKFB3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PFKFB3 (PFKFB3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PFKFB3 gRNA (BRDN0001147212)

    Plasmid

    #76463

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PFKFB3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PFKFB3 (PFKFB3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. STK4 gRNA (BRDN0001149002)

    Plasmid

    #76074

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK4 (STK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAPK3 gRNA (BRDN0001149014)

    Plasmid

    #75966

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge