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Plasmid#168517PurposeGFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.DepositorInsertspTorA-GFP-H6C
UseTags6xHisC tag and spTorA (signal peptide of E. coli …ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM S297A
Plasmid#169983PurposeTet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, S297APromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
rUBR5_2383-2442_pGEX6P1
Plasmid#209791PurposeBacterial expression of UBR5 Mlle domainDepositorInsertMlle domain of UBR5 (Ubr5 Rat)
UseTagsGSTExpressionBacterialMutationaa 2383-2442 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM L368G
Plasmid#169985PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, L368GPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM R394E
Plasmid#169987PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, R394EPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM F405G
Plasmid#169988PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, F405GPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM F370G
Plasmid#169986PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, F370GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM E274R
Plasmid#169984PurposeTet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND F405G + S297A
Plasmid#169974PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, F405GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND E274R + S297A
Plasmid#169968PurposepDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E + S297A
Plasmid#169972PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, R394EPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM E274R
Plasmid#169977PurposepDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A
Plasmid#169955PurposeMammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationS297A, R394EPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A
Plasmid#169957PurposeMammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationS297A, F405GPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A
Plasmid#169951PurposeMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationE274R, S297APromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 neg ctrl
Plasmid#112030PurposeMode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
VP16-ER beta (long)
Plasmid#11352DepositorInsertER beta long (ESR2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-spTorA-GFP(C)
Plasmid#169041PurposeGFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.DepositorInsertH6-spTorA-GFP
UseTags6xHis, TEV cleavage site, and spTorA (signal pept…ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NPY1.0
Plasmid#208675PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cellsDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NPY1.0
Plasmid#208676PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neuronsDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only