We narrowed to 992 results for: dTomato

1. pAAV-rBrokenheart

   Plasmid

   #203394

   Purpose

   AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   Brokenheart
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFIN-XOPS-tdTOM-MOPS-GFP

   Plasmid

   #44347

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Inserts

   XOPS-tdTOM
   MOPS-GFP
   

   Use

   Lentiviral

   Promoter

   Xenopus Opsin (XOPS) (pos 3131-4495) and mouse op…

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMADM-alpha

   Plasmid

   #36890

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   Neo
   ATG (T)
   GFP
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, deleted…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. eMscL WT

   Plasmid

   #107454

   Purpose

   eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

   Depositor

   Francesco Difato

   Article

   Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

   Insert

   bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
   

   Use

   Adeno-associated viral

   Tags

   Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

   Expression

   Mammalian

   Promoter

   human synapsin 1

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pCAG-mRuby3-WPRE

   Plasmid

   #107744

   Purpose

   Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

   Depositor

   Rylan Larsen

   Article

   Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. UPB-esophagus (UPB5)

   Plasmid

   #180783

   Purpose

   Unique Projection Barcode (UPB5)-Esophagus for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM3Dq ( partial ) (CHRM3 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-short_BrokenHeart (no BC)

   Plasmid

   #86950

   Purpose

   AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   Brokenheart construct
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBa-flag-BicD2 594-FKBP

   Plasmid

   #64206

   Purpose

   Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Bicaudal D protein (Bicd2 Mouse)
   

   Tags

   FKBP and flag

   Expression

   Mammalian

   Mutation

   no start methionine, aa1-594

   Promoter

   chicken Beta Actin

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDragon-Ctgf

   Plasmid

   #155015

   Purpose

   For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

   Depositor

   Alberto Rosello-Diez

   Article

   Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

   Insert

   Ccn2 (Ccn2 Mouse)
   

   Use

   Mouse Targeting

   Mutation

   M24T (please see depositor comments)

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-tdPCP-CIBN

    Plasmid

    #183937

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-CIBN (CIB1 Synthetic, Mustard Weed)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177365

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Promoter

    CMV and U6 promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2705-AGER-eGFP

    Plasmid

    #216474

    Purpose

    donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    eGFP
    

    Use

    CRISPR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNOC_dCas9_sgRNA Td1

    Plasmid

    #176257

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid

    #198753

    Purpose

    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

    Depositor

    Jacco van Rheenen

    Article

    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

    Insert

    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDragon-p21

    Plasmid

    #155016

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-PSD95-EGFP-WPRE

    Plasmid

    #133785

    Purpose

    Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    PSD95-EGFP (Dlg4 Rat)
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. eMscL G22S

    Plasmid

    #107455

    Purpose

    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

    Depositor

    Francesco Difato

    Article

    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

    Insert

    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    

    Use

    Adeno-associated viral

    Tags

    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

    Expression

    Mammalian

    Mutation

    Bearing a glycine to serine substitution at posit…

    Promoter

    human synapsin 1

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT272_(pRosa26-GTET)

    Plasmid

    #36882

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    diphteria toxin A
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. INS-GFP-CD19-EPT

    Plasmid

    #210469

    Purpose

    Donor plasmid for knock-in GFP-CD19 into the human INS locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36881

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge