We narrowed to 1,121 results for: gibson assembly
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Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pMMK805
Plasmid#207139PurposeAssembled Tn10 with ptac promoter driving gfpDepositorInsertTn10-Ptac-GFP
UseSynthetic BiologyExpressionBacterialPromoterptacAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMulti-sgRNA-LacZ-DsRed
Plasmid#99914PurposeReceptor plasmid for the assembly of multiple sgRNAs with DsRed coexpressionDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBD21_037
Plasmid#109154PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720DepositorInsertsmRFP
iRFP720
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMulti-sgRNA-LacZ
Plasmid#99913PurposeReceptor plasmid for the assembly of multiple sgRNAsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_034
Plasmid#109151PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFPDepositorInsertsmRFP
TagBFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH512
Plasmid#102666PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH511
Plasmid#102665PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNA-Z
Plasmid#238995PurposeNode A carrying sgRNA-2 that can be used for the assembly of pEND-Z11 or pEND-ZYDepositorInsertsgRNA-2
UseSynthetic BiologyExpressionBacterialMutationWTAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA-Y
Plasmid#238993PurposeNode A carrying sgRNA-1 that can be used for the assembly of pEND-Y11DepositorInsertsgRNA-1
UseSynthetic BiologyExpressionBacterialMutationWTAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-BFP-NLS-P2A-RSV P-1xALFATag@72AA
Plasmid#246864PurposeExpress BFP-NLS-P2A-RSV P-1xALFATag@72AA (Pexo-ALFATag) for RSV imaging.DepositorInsertBFP-NLS-P2A-RSV P-1xALFATag@72AA
UseLentiviralTagsBFP-NLSExpressionMammalianMutationRSV phosphoprotein (P) contains a single ALFA Tag…PromoterSFFVAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen
Plasmid#196974PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tagDepositorAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-BFP-NLS-P2A-RSV P-1xSunTag@72AA
Plasmid#246863PurposeExpress BFP-NLS-P2A-RSV P-1xSunTag@72AA (Pexo-SunTag) for RSV imaging.DepositorInsertBFP-NLS-RSV P-1xSunTag@72AA
UseLentiviralTagsBFPExpressionMammalianMutationInserted a single SunTag copy at amino acid 72 of…PromoterSFFVAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHR-HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag
Plasmid#246867PurposeExpress HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag (M2-1exo-SunTag) for RSV imaging.DepositorInsertHaloTag-P2A-RSV M2-1-C-terminal-1xSunTag
UseLentiviralTagsHaloTagExpressionMammalianMutationThe RSV M2-1 protein with a C-terminal insertion …PromoterSFFVAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHR-HaloTag-P2A-RSV N-1xSunTag@105AA
Plasmid#246868PurposeExpress HaloTag-P2A-RSV N-1xSunTag@105AA (Nexo-SunTag) for RSV imaging.DepositorInsertHaloTag-P2A-RSV N-1xSunTag@105AA
UseLentiviralTagsHaloTagExpressionMammalianMutationThe RSV nucleoprotein (N) with an insertion of a …PromoterSFFVAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKG1334_pHarbor-PiggyBac_backbone
Plasmid#239706PurposePaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated intDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-sgGOT1
Plasmid#72874PurposeThe lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).DepositorInsertsgGOT1
UseLentiviralAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only