We narrowed to 5,366 results for: pcDNA3.1

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pcDNA3.1_mGFP-FGFR3-K650E

Plasmid

#191757

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1948A>G (p.K650E) + silent mutation c.1938C&…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) Hu 4-1BB(CD137)-Fc

Plasmid

#183251

Purpose

Soluble expression of human 4-1BB (CD137)-Fc. Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

41BB Fc

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR(T/A)

Plasmid

#118406

Purpose

Negative-control mutant for ExRai-AKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS3

Plasmid

#104353

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Mutation

AA1-733 ITGB2 present

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

Plasmid

#159132

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11F

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-WT

Plasmid

#92009

Purpose

Expresses Lambda-N-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-HDD

Plasmid

#52063

Purpose

It encodes a mutant of TTF-1 in which the homeodomain is deleted

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Expression

Mammalian

Mutation

Homeodomain deleted

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7A-mGFP

Plasmid

#62633

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RaACR_687-EYFP

Plasmid

#111077

Purpose

Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RaACR_687

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 2+linker3

Plasmid

#176920

Purpose

express Granulin 2 with linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2+linker3 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-alpha4 wt (Hygro)

Plasmid

#80017

Purpose

Mammalian expression of human integrin alpha4, Hygro selection

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPE-600n

Plasmid

#13541

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pY120 (pcDNA3.1-huFbCpf1)

Plasmid

#92296

Purpose

Expression of humanized FbCpf1

Depositor

Feng Zhang

Insert

huFbCpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236A-pcDNA3.1

Plasmid

#85764

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236A

Promoter

CMV

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236S-pcDNA3.1

Plasmid

#85763

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236S

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Rat NCX1.3 (pcDNA3.1+)

Plasmid

#75191

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROR1-V5/HIS

Plasmid

#205607

Purpose

Expression of human ROR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR1 receptor tyrosine kinase (ROR1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-170nDelta13V

Plasmid

#18018

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16F Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

WT binding protein (no F16A)

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Rab10-FLAG

Plasmid

#135559

Purpose

Express mouse Rab10 in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Rab10 (Rab10 Mouse)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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