We narrowed to 13,211 results for: CAR

1. pOTTC763 - pX458 with rat Rosa26 gRNA A

   Plasmid

   #113161

   Purpose

   A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat Rosa26
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-iSeroSnFR-Nlgn

   Plasmid

   #128485

   Purpose

   Fluorescent reporter for serotonin (dendrite localization tag)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Has Service

   AAV9

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Full-length neuroligin, Ig-kappa leader, and cpsf…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 Sox2 3H b

   Plasmid

   #26352

   Depositor

   Matthew Meyerson

   Article

   Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

   Insert

   Sox2 shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

4. 6xHis-MBP-BrCas12b-FNLDTA

   Plasmid

   #195340

   Purpose

   Expresses Engineered BrCas12b in Bacteria

   Depositor

   Piyush Jain

   Article

   Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis Tag and MBP, TEV cleavage site, 6x His Tag

   Expression

   Bacterial

   Mutation

   F208W, N524V, L795I, D868V, T874S, A1015E

   Promoter

   T7 Promoter

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-AlkB

   Plasmid

   #228218

   Purpose

   Express wild type AlkB protein in E. coli BL21(DE3)

   Depositor

   Junchao Shi

   Article

   Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

   Insert

   Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   codon optimized

   Promoter

   T7

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ad:2CMV_A2R2-N25Ctr

   Plasmid

   #122243

   Purpose

   Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

   Depositor

   Corey Neu

   Article

   Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

   Inserts

   C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
   Actinin alpha 2 (Actn2 Mouse)
   

   Use

   Adenoviral

   Tags

   Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

   Expression

   Mammalian

   Mutation

   deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

   Promoter

   CMV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-Flag-CDK8-W105M-IRES-mCherry

   Plasmid

   #68857

   Purpose

   Expresses Human CDK8 W105M Mutant

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 8 (CDK8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W105M

   Promoter

   EF1α

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_HA.V5.FLAG_NGFR

   Plasmid

   #158250

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.V5.FLAG

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLED.NPL

   Plasmid

   #193014

   Purpose

   Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330-Axin1

    Plasmid

    #162543

    Purpose

    sgRNA targeting Axin1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Axin1 (Axin1 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-RIPK2

    Plasmid

    #23846

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RIPK2 (RIPK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid

    #111851

    Purpose

    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G13D (KRAS Human)
    

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Changed Glycine 13 to Aspartic Acid

    Promoter

    T5

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1α1.1-Jaws-KGC-GFP-ER2

    Plasmid

    #153537

    Purpose

    AAV-mediated expression of Jaws-GFP-ER2 under the EF1α promoter (1.1kb short version).

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    EF1α1.1

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-Jaws-KGC-GFP-ER2

    Plasmid

    #99233

    Purpose

    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter. Using bGH pA signal.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CAG

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

    Plasmid

    #132667

    Purpose

    Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)
    

    Expression

    Mammalian

    Promoter

    4xHRE_YB TATA

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB-Tet-On-HTR6-RhoA sensor

    Plasmid

    #189614

    Purpose

    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6 (HTR6 Human)
    

    Tags

    GFP and mScarlet

    Expression

    Mammalian

    Promoter

    Tet3G

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1α1.1-ChrimsonR-GFP

    Plasmid

    #122063

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV2

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1a（1.1kb short version)

    Available Since

    March 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-EF1A-CCNE2

    Plasmid

    #162554

    Purpose

    Overexpression of CCNE2

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE2 (CCNE2 Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid

    #114069

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-syn-AICD-IRES-hrGFP

    Plasmid

    #107548

    Purpose

    high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD (APP Human)
    

    Use

    AAV

    Mutation

    None

    Promoter

    human synapsin

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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