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pCgVchDonor

Plasmid

#203817

Purpose

DNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coli

Depositor

Sheng Yang

Publication

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

Mini-Tn, Spec cassette

Use

CRISPR

Expression

Bacterial

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pINO4-NLS-Pfv-Sapphire-HO

Plasmid

#203660

Purpose

Yeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.

Depositor

Liam Holt

Publication

Shu T et et al bioRxiv 2022

Insert

PfV

Use

CRISPR

Tags

Sapphire

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn1-mClover3/CAAX

Plasmid

#197591

Purpose

Lentiviral transfer vector for the neuronal expression of membrane-bound mClover3 (good visualization of neuronal processes)

Depositor

Patrik Verstreken

Insert

mClover3-CAAX

Use

Lentiviral

Tags

CAAX

Promoter

hSynI

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.6

Plasmid

#197199

Purpose

Expresses the protein of BDFP1.6 in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.6

Use

AAV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCardinal

Plasmid

#197195

Purpose

Expresses the protein of mCardinal in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal

Use

AAV

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJLG029

Plasmid

#192980

Purpose

BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification

Depositor

Clay Fuqua

Publication

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

pruR-3xFLAG

Tags

3XFLAG

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY51

Plasmid

#191054

Purpose

mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mec-3 (mec-3 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EcAvs4

Plasmid

#188794

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Publication

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Expression

Bacterial

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT3A

Plasmid

#183283

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT3A

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP1

Plasmid

#183323

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP1

Use

Lentiviral

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRA

Plasmid

#183313

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRA

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PARP1

Plasmid

#183312

Purpose

All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PARP1

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH1

Plasmid

#183300

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH1

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC2

Plasmid

#183298

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC2

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-U63-QtgRNA2.1-8R

Plasmid

#170514

Purpose

gRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

Depositor

Chun Han

Publication

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCVD208

Plasmid

#159762

Purpose

Expression vector for wild-type Fc-fused ACE2(740)

Depositor

Jim Wells

Publication

Glasgow et al Proc Natl Acad Sci U S A. 2020 Nov 10;11

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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AL12R

Plasmid

#161007

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Publication

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Tags

PP7 and H2B-mScarlet

Expression

Plant

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Publication

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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