We narrowed to 11,847 results for: NSI

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pCMV-3×FLAG-SLC35A1

Plasmid

#186282

Purpose

transient expression of N-terminal 3×FLAG tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Tags

3×FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pT2iU6m5Hoxa13bE4PCh(#765)

Plasmid

#184102

Purpose

cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis

Depositor

Sophie Vriz

Insert

5myc-ZfHoxa13b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes) + in vitro tra…

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi+SP6

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

Plasmid

#162802

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pICH78133

Plasmid

#50292

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSB4C5_pBen-BenR

Plasmid

#128127

Purpose

Expressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

BenR

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBen

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2+NtEUt

Plasmid

#170883

Purpose

Phytobrick - NtEUt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

NtEUt

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVEG17*

Plasmid

#111856

Purpose

Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

Depositor

Nikhil Nair

Article

Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

Insert

GAL1p-GAL3-Syn4.1-TEFt

Use

Synthetic Biology

Expression

Yeast

Mutation

D68N, V69M, A109V, I271L

Promoter

GAL1p

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pXW101Plux2-gfp

Plasmid

#160819

Purpose

AHL-responsive single-layered circuit

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23101, Plux2

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-Sesn3

Plasmid

#61869

Purpose

Transient overexpression of Flag-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
CD4_LD 0_TEV

Plasmid

#58870

Purpose

MESA protease chain with CD4 ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with CD4 ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pTEI134

Plasmid

#126071

Purpose

Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp7-10

Plasmid

#72742

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 xIGF2

Plasmid

#24689

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Insulin-like growth factor 2 (igf2-b Frog)

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pDL12c

Plasmid

#17835

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, argB_1626,

Use

Synthetic Biology; Aspergillus nidulans expression

Mutation

The argB_1626 mutation consists of two sequential…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pES001 (pQacA-deGFP)

Plasmid

#69030

Purpose

Reporter Plasmid - deGFP driven by a qacR responsive promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

deGFP

Expression

Bacterial

Promoter

qacA

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAG142 TNS3 3'UTR

Plasmid

#12000

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N7 3'UTR (TNS3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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