We narrowed to 11,712 results for: NSI

1. pJexpress414_OmpA171_R4cons_Y129H

   Plasmid

   #64302

   Purpose

   Conservative version of OmpA Redesign 4 with Y129H mutation

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA_R4cons_Y129H
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Mutation

   25 mutations to lipid-facing surface

   Promoter

   T7

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. piHGpd/G170D

   Plasmid

   #1328

   Depositor

   Susan Lindquist

   Article

   Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

   Insert

   GPD promoter-hsp82 ts mutant (G170D) (HSP82 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   G170D

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-roxSTOProx-ZsGreen

   Plasmid

   #51274

   Purpose

   Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   roxSTOProx-ZsGreen
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGilesSHdI_psmyc_His6_triple red

   Plasmid

   #170047

   Purpose

   Mono-Responsive Reporter Gene Constructs for triple red

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mScarlet
   COmScarlet
   mRuby3
   

   Use

   Unspecified

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5HT6-mCherry-G-GECO1.0

   Plasmid

   #47500

   Purpose

   Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   G-GECO1.0
   

   Tags

   5HT6 and mCherry

   Expression

   Mammalian

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAUX_OL

   Plasmid

   #166246

   Purpose

   use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
   

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and BBa_J23116

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flamindo2

   Plasmid

   #73938

   Purpose

   Expresses biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   Flamindo2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbS5c-mCherry-AsLOV2*(543)

   Plasmid

   #202712

   Purpose

   Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Insert

   mCherry-AsLOV2*(543)
   

   Expression

   Bacterial

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH3.2_mTagBFP2-MS2x12

    Plasmid

    #205558

    Purpose

    Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-Dre-T2A-TagRFP

    Plasmid

    #167968

    Purpose

    Transient expression of Dre and TagRFP

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    Dre recombinase
    

    Tags

    TagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH2.22_mCherry-MS2x8

    Plasmid

    #205537

    Purpose

    Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSR58.6

    Plasmid

    #63176

    Purpose

    Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTcI42-Wasabi

    Plasmid

    #86118

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI42
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, K6N, S33T, Y61H, L119P, F122C

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP.N3-SLC35A1-GFP

    Plasmid

    #186281

    Purpose

    transient expression of C-terminal GFP tagged SLC35A1 isoform a in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

    Insert

    SLC35A1 (SLC35A1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTcI-Wasabi

    Plasmid

    #86101

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)

    Plasmid

    #70745

    Purpose

    for building 3-color reporter plasmid

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    mCherry-ssrA
    

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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