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Plasmid#223811PurposeIVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.DepositorInsertJarid2 (Jarid2 Mouse)
UseIn vitro transcriptionTagsFlagExpressionMammalianMutationPromoterCMV, T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP WPRE bGH pA (JDW 488)
Plasmid#224540PurposeA Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleusDepositorInsertnls-EGFP-WPRE-bGH-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME nls BFP FLAG WPRE bGH pA (JDW 484)
Plasmid#224539PurposeA Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleusDepositorInsertnls-mTagBFP2-FLAG_WPRE-bGH-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-10His-FKBP(f36v)-SNAP
Plasmid#200282PurposeFor recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion proteinDepositorInsertFKBP-SNAPtag
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(MGD) (SB264)
Plasmid#227613PurposeInducible expression of Strep-tagged Opa1(MGD) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-580 (MGD) and aa 938-960PromoterT7Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-pSu9-HaloTag7-SNAP
Plasmid#217319PurposeExpresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.DepositorInsertNeurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
UseRetroviralTagsHaloTag and SNAPExpressionMutationPromoterCMVAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHsp70(K71E)-GFP
Plasmid#228196Purposeexpressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tagDepositorInsertHSP70(K71E) (HSPA1A Human)
UseTagsGFPExpressionMammalianMutationK71EPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TadA-8e-eIscBn
Plasmid#227018PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoterDepositorInsertbpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
UseTagsExpressionMutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
IscB-v3
Plasmid#227015PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB driven by CMV promoterDepositorInsertSV40 NLS-OgeuIscB-v3-nucleoplasmin NLS
UseTagsExpressionMutationD96R/E84R/V159RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
eIscB
Plasmid#227016PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fusion with extra SV40 NLS driven by CMV promoterDepositorInsertSV40 NLS-OgeuIscB-v3-SV40-nucleoplasmin NLS
UseTagsExpressionMutationD96R/E84R/V159RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC023 (∆MBH)
Plasmid#226201PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).DepositorInsertHomology arms for membrane bound hydrogenase operon
UseSynthetic BiologyTagsExpressionMutation∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPICZ A-SLC25A46-His (SB250)
Plasmid#227599PurposeInducible expression of His-tagged SLC25A46 in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
UseTags10xHisExpressionYeastMutationPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1(MGD) (SB252)
Plasmid#227601PurposeInducible expression of Strep-tagged Opa1(MGD) in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionYeastMutationcontains aa 253-580 (MGD) and aa 938-960PromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)
Plasmid#227614PurposeOverexpression of SLC25A46 in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
UseTagsP2A-mElectra1ExpressionMammalianMutationPromoterCMV, T7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
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p3E-WPRE-bGH-pA (JDW 1221)
Plasmid#224527PurposeA Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRNDepositorInsertWPRE-bGH
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only