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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9881 - 9900 of 11338 results

  9. pMCh-1538

    Plasmid
    #82431
    Purpose
    Plasmid for expression of NHA-tagged human-Nematostella GW182 11W11A chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 11W11A chimera
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    CMV
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82431-plasmid-map-sequence-id-156186

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  10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

    Plasmid
    #217799
    Purpose
    Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217799-plasmid-map-sequence-id-432866

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  18. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  20. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    atf4 (ATF4 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226105-plasmid-map-sequence-id-448414

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Showing: 9881 - 9900 of 11338 results