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pET-51b(+)-Caprola_02

Plasmid

#194663

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_02 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_02

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_03

Plasmid

#194664

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_03 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_03

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP

Plasmid

#205704

Purpose

Gal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila flies

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola5-EGFP

Expression

Insect

Promoter

20 Gal4

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-IG-ATG3 (H262A)

Plasmid

#212027

Purpose

Expresses untagged ATG3 (H262A) in mammalian cells

Depositor

Michael Lazarou

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-mCherry

Plasmid

#210377

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3C (ORF1ab SARS-CoV-2)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MAFG

Plasmid

#207817

Purpose

N-terminal tagged MAFG for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-MAFG

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746

Plasmid

#207819

Purpose

N-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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SEC61B-2Strep

Plasmid

#210375

Purpose

Expresses SEC61B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SEC61B (SEC61B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM106B-2Strep

Plasmid

#210360

Purpose

Expresses TMEM106B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-NS-A

Plasmid

#207823

Purpose

Dual expression of Cas9 and non-specific sgRNA

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

non-specific sgRNA

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EF-1a; U6

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-NS-B

Plasmid

#207824

Purpose

Dual expression of Cas9 and non-specific sgRNA

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

non-specific sgRNA

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EF-1a; U6

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-ZNF746-V5-miniTurbo

Plasmid

#207820

Purpose

C-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-V5-miniTurbo (ZNF746 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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ALIX(P801G)-mNeonGreen

Plasmid

#191192

Purpose

Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (P801G) (PDCD6IP Synthetic, Human)

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Pro 801 mutated to Gly

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-ATPIF1 E55A

Plasmid

#204704

Purpose

expresses ATPIF1 E55A in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)

Expression

Mammalian

Mutation

E55A

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-NeoCOVGT3-d2EGFP

Plasmid

#201446

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT3_d2EGFP

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT1-d2EGFP

Plasmid

#201447

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT1_d2EGFP

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT2-d2EGFP

Plasmid

#201448

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT2_d2EGFP

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT4-d2EGFP

Plasmid

#201449

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT4_d2EGFP

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Y118D)

Plasmid

#188638

Purpose

For expression of human ATG13(Y118D)

Depositor

Michael Lazarou

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Changed tyrosine 118 to aspartic acid

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(W50D)

Plasmid

#188639

Purpose

For expression of human ATG13(W50D)

Depositor

Michael Lazarou

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Changed tryptophan 50 to aspartic acid

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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