We narrowed to 10,989 results for: T7
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Plasmid#177363PurposeExpresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tagDepositorInsertKIF1A (KIF1A Human)
TagsHis tag and Leucine zipperExpressionBacterialMutationchanged Proline 305 to LeucinePromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)_co_L-HicDH
Plasmid#185453PurposeCDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).DepositorInsertL-HicDH from Lactobacillus confusus DSM 20196
ExpressionBacterialPromoterT7Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-GB1-EWS_171-264
Plasmid#180466PurposeExpresses the construct His8-GB1-EWS_171-264 with a TEV cleavage site between GB1 and EWS_171-264DepositorInsertEWSR1 (EWSR1 Human)
TagsGB1 and His8ExpressionBacterialMutationdeleted 1-170, 265-656PromoterT7Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-GFP-EWS_1-120
Plasmid#180464PurposeExpresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120DepositorInsertEWSR1 (EWSR1 Human)
TagsGFP and His8ExpressionBacterialMutationdeleted 121f-656PromoterT7Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-sumo-EWS_91-199
Plasmid#180465PurposeExpresses the construct His8-sumo-EWS_91-199DepositorInsertEWSR1 (EWSR1 Human)
TagsHis8 and SUMOExpressionBacterialMutationdeleted 1-90, 200-656PromoterT7Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP2
Plasmid#183771PurposeExpresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP2 (INPPL1 Human)
Tags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa18 to aa985 onlyPromoterT7 lacAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL003
Plasmid#180607PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertionDepositorInsertDpbCasX with R3 loop
UseCRISPRTags10x His and MBPExpressionBacterialPromoterT7Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-390)-H6
Plasmid#183781PurposeExpress PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (w Fly)
Tags6 His tag at the C-terminusExpressionBacterialMutation6 His tag at the C-terminusPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PC(1-86)-H6
Plasmid#183780PurposeExpress PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assaysDepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_DCUN1D1_PONY
Plasmid#178473PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-CXCL9-mCherry-SBP
Plasmid#170678PurposeExpresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsTagsSBP and mCherryExpressionMammalianMutationChanged threonine 23 to glutamate (T23E)PromoterIRES and T7Available SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066
Plasmid#175586Purposein vitro transcription of mut RBM8A 3'UTRDepositorInsertRBM8A-3'UTR AluY-Sz mut 2598-4066
UseIn vitro transcriptionPromoterT7Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD400
Plasmid#163107PurposeExpression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_eYFP_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD188
Plasmid#163099PurposeExpression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mEos3.2_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1-4H pET151
Plasmid#159386PurposeExpresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
Tagshis-tag, TEV cleavage siteExpressionBacterialMutationTLNRD1 4-helix domain (residues 143-273)PromoterT7Available SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-His D592Amb
Plasmid#169022PurposeExpresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteriaDepositorInsertp97
TagsHisExpressionBacterialMutationD592TAGPromoterT7Available SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-Strep-His E314Amb
Plasmid#169021PurposeExpresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteriaDepositorInsertp97
TagsHis and SBPExpressionBacterialMutationE314TAGPromoterT7Available SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/R
Plasmid#166976PurposeExpresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only