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Addgene

We narrowed to 157 results for: SPT

Showing: 121 - 150 of 157 results
  1. Affibody HER2:243.BirA*-pET301/NT-DEST

    Plasmid
    #174692
    Purpose
    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*
    Depositor
    Insert
    Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
    Use
    Tags
    HIS tag
    Expression
    Bacterial
    Mutation
    R118G mutation in BirA
    Promoter
    T7 promoter
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid
    #174691
    Purpose
    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*
    Depositor
    Insert
    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    Use
    Tags
    HIS tag
    Expression
    Bacterial
    Mutation
    R118G mutation in BirA
    Promoter
    T7 promoter
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. MFE23-ZZ

    Plasmid
    #223553
    Purpose
    MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.
    Depositor
    Insert
    pET302-MFE23-ZZ
    Use
    Tags
    HA tag and HIS tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pAVA-Gal11p-LGF2(fs)

    Plasmid
    #127482
    Purpose
    Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Insert
    Gal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    LGF2 is frame shifted by the insert of one nucleo…
    Promoter
    Available since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid
    #127483
    Purpose
    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Insert
    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    One nucleotide inserted between lambda CI and LGF…
    Promoter
    Available since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Insert
    Spike Protein
    Use
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. AP2u2-CMV-Ace2-IgG1

    Plasmid
    #226455
    Purpose
    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification
    Depositor
    Inserts
    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    Use
    Tags
    6xHis Tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 150 of 157 results