Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 772 results for: tev cleavage

Showing: 161 - 200 of 772 results

  1. pAAP2

    Plasmid
    #171208
    Purpose
    bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    pgl-3 (pgl-3 Nematode)
    Use
    Tags
    MBP::HIS::TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171208-plasmid-map-sequence-id-335799

    Enlarge

  2. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72473-plasmid-map-sequence-id-132656

    Enlarge

  3. pst39-NST-clone14

    Plasmid
    #133421
    Purpose
    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
    Depositor
    Gerhard Wagner
    Insert
    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ORF for Nip1 encodes aa 4-156 (not complete ORF),…
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133421-plasmid-map-sequence-id-267716

    Enlarge

  5. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

    Enlarge

  6. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

    Enlarge

  7. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

    Enlarge

  9. thoc5.018.389

    Plasmid
    #137266
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    thoc5 (THOC5 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    018-389
    Promoter
    Available since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    137266-plasmid-map-sequence-id-323966

    Enlarge

  10. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

    Enlarge

  12. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

    Enlarge

  13. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

    Enlarge

  14. pET mRuby2 LIC cloning vector (H6-mRuby2)

    Plasmid
    #86934
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mR…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86934-plasmid-map-sequence-id-174750

    Enlarge

  15. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

    Enlarge

  16. pET mClover LIC cloning vector (H6-mClover)

    Plasmid
    #86931
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mC…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86931-plasmid-map-sequence-id-174751

    Enlarge

  17. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

    Enlarge

  18. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

    Enlarge

  19. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

    Enlarge

  20. pSUMO-HsSyx3

    Plasmid
    #179288
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179288-plasmid-map-sequence-id-354924

    Enlarge

  21. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Use
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111274-plasmid-map-sequence-id-215709

    Enlarge

  22. pET MBP mClover LIC cloning vector (MBP-mClover)

    Plasmid
    #86930
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86930-plasmid-map-sequence-id-174752

    Enlarge

  23. pET MBP tdTomato LIC cloning vector (MBP-tdTomato)

    Plasmid
    #86927
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86927-plasmid-map-sequence-id-181702

    Enlarge

  24. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72456-plasmid-map-sequence-id-133910

    Enlarge

  25. pEGGsH6_MCS_SHORT_outFrame

    Plasmid
    #211439
    Purpose
    Vector backbone for random polymerase chain reaction screen
    Depositor
    Helmut Pospiech
    Insert
    MCS-tev cleavage site-EGFP-6xHis-Tag
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    211439-plasmid-map-sequence-id-421993

    Enlarge

  26. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

    Enlarge

  27. pCAGEN_NES_V5_GFP_FTA

    Plasmid
    #235633
    Purpose
    Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    GFP
    Use
    Tags
    FLAG-TEV cleavage site-AviTag and NES-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235633-plasmid-map-sequence-id-467518

    Enlarge

  28. pMtb MCM

    Plasmid
    #138296
    Purpose
    Expression of Mtb MCM small and large subunit
    Depositor
    Ruma Banerjee
    Article
    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d
    Inserts
    mutA
    mutB
    Use
    Tags
    His tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    inserted TEV cleavage site between His-tag and m…
    Promoter
    T7
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    138296-plasmid-map-sequence-id-292603

    Enlarge

  29. pET-30a(+)-N-SH2+IA

    Plasmid
    #111273
    Purpose
    express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Use
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111273-plasmid-map-sequence-id-215706

    Enlarge

  30. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid
    #86933
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86933-plasmid-map-sequence-id-174748

    Enlarge

  31. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

    Enlarge

  32. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

    Enlarge

  33. pEGGsH6-MCS_SHORT_inFrame

    Plasmid
    #211438
    Purpose
    Vector backbone for random polymerase chain reaction screen
    Depositor
    Helmut Pospiech
    Insert
    MCS-tev cleavage site-EGFP-6xHis-Tag
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    211438-plasmid-map-sequence-id-420494

    Enlarge

  34. pMBP-HsSyx3

    Plasmid
    #179287
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179287-plasmid-map-sequence-id-354925

    Enlarge

  35. pMBP-HsSyx-375-792

    Plasmid
    #179291
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179291-plasmid-map-sequence-id-354915

    Enlarge

  36. 10279-E06

    Plasmid
    #234311
    Purpose
    Gateway ORF Entry clone of human TGFBR2 iso1 with stop codon (for native or N-terminal fusions); N-terminal tev cleavage site and E526Q mutation
    Depositor
    Dominic Esposito
    Insert
    tev-Hs TGFBR2 iso1 E526Q (TGFBR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    E526Q
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234311-plasmid-map-sequence-id-468861

    Enlarge

  37. 10279-E07

    Plasmid
    #234312
    Purpose
    Gateway ORF Entry clone of human TGFBR2 iso1 with stop codon (for native or N-terminal fusions); N-terminal tev cleavage site and R537P mutation
    Depositor
    Dominic Esposito
    Insert
    tev-Hs TGFBR2 iso1 R537P (TGFBR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    R537P
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234312-plasmid-map-sequence-id-469767

    Enlarge

  38. pMLLAC-J72041

    Plasmid
    #26186
    Depositor
    Christopher Anderson
    Insert
    Tev Protease Cleavage site
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    26186-plasmid-map-sequence-id-12307

    Enlarge

  39. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72347-plasmid-map-sequence-id-133755

    Enlarge

  40. pHLmMBP-11

    Plasmid
    #72349
    Purpose
    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72349-plasmid-map-sequence-id-133760

    Enlarge

Showing: 161 - 200 of 772 results