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Plasmid#66083PurposeG protein alpha-s internally tagged with EGFP and EE epitopeDepositorInsertG-alpha-s-EE-EGFP (Gnas Rat)
UseTagsEGFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCherry
Plasmid#66968PurposeG protein alpha-s internally tagged with mCherry and EE epitopeDepositorInsertG-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha- s…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-CFP
Plasmid#55793PurposeG protein alpha-s internally tagged with ECFP and EE epitopeDepositorInsertG-alpha-s-EE-ECFP (Gnas Rat)
UseTagsECFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationHis was substituted for Asn164 in ECFP (Clontech)…PromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCerulean
Plasmid#66084PurposeG protein alpha-s internally tagged with mCerulean and EE epitopeDepositorInsertG-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro
Plasmid#192683PurposeLentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ1-LSS-mKATE2(N)
Plasmid#207159PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG1
UseTagsExpressionMammalianMutationN-terminally tagged with LSS-mKATE2PromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ2-LSS-mKATE2(N)
Plasmid#207160PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG2 (GNG2 Human)
UseTagsExpressionMammalianMutationN-terminally tagged with LSS-mKATE2PromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
G15-CASE
Plasmid#168126PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at E244/N245 within GNA15PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi2-CASE
Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA
Plasmid#136537PurposeLentiviral expression vector for an inducible BirADepositorInsertBirA
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA
Plasmid#136521PurposeUsed as a donor vector to clone into pSLIKDepositorInsertBirA
UseEntry vector for gateway cloningTagsExpressionMutationR118G (highly promiscuous form)PromoterAvailable sinceJune 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRC2_3xflag_Luciferase
Plasmid#136519PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertLuciferase
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTI-2A.pMIG II
Plasmid#52111Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsT2A (from Thosea asigna virus)ExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_Luciferase
Plasmid#136528PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_Zeo_3xflag_Luciferase
Plasmid#136533PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLXSN_HPV16E7∆DLYC
Plasmid#136588PurposeRetroviral vector for expression of RB-binding mutant of HPVE7DepositorInsertHPV16E7∆DLYC
UseRetroviralTagsExpressionMutationdel aa 21-24, full length is 1-98PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shLuc
Plasmid#136587PurposeExpresses an inducible short hairpin targeting firefly luciferase sequenceDepositorInsertshLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA_NRF2
Plasmid#136524PurposeUsed as a donor vector to clone into pSLIKDepositorInsertNRF2 (NFE2L2 Human)
UseEntry vector for gateway cloningTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2
Plasmid#136579Purposemammalian expression of mRFP1-tagged human NRF2DepositorInsertNRF2 (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only