We narrowed to 594 results for: bira

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEHAC1- HA-BirA-Traf6

Plasmid

#232585

Purpose

Express BirA tagged to the N-terminus of TRAF6.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA -TRAF6 (TRAF6 Human)

Tags

HA

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-BirA-HA-Tulp3

Plasmid

#236749

Purpose

Express BirA and HA tagged TULP3 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

Insert

Tulp3 (Tulp3 Mouse)

Tags

BirA and HA

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pOz-humBirA-GFP

Plasmid

#133283

Purpose

Expression of BirA-GFP fusions in mammalian cells

Depositor

Arman Kulyyassov

Article

Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

Insert

eGFP

Use

Retroviral

Tags

Humanized wild type biotin ligase BirA

Expression

Mammalian

Promoter

MoMuLV

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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BirA in pENTR1A (w860-1)

Plasmid

#29645

Depositor

Eric Campeau

Insert

BirA

Use

Entry vector

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_RACK1:1-317

Plasmid

#210889

Purpose

Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUAS-Myc-BirA-G3-ER

Plasmid

#173815

Purpose

Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Depositor

Norbert Perrimon

Article

Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

Insert

Myc-BirA-G3-ER

Tags

Myc

Expression

Insect

Promoter

UAS

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry-pBirA-HA

Plasmid

#154458

Purpose

expresses mCherry-pBirA-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry-pBirA-HA

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET22b-BirA-CPDSalI

Plasmid

#38258

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

birA (birA E. coli)

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pGEX4T1-BirA

Promoter

T7

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
pMTB2-cytoBirA-2A-mCherry_Ras

Plasmid

#80066

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Beta-actin2

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-BirA

Plasmid

#71752

Purpose

Aurein1.2+36GFP-BirA fusion for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2+36GFP-BirA

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-Puro-BirA

Plasmid

#246557

Purpose

Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

BirA

Use

Retroviral

Tags

FLAG

Promoter

MMLV LTR

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3

Plasmid

#240233

Purpose

Expression of Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

Myc-BirA*G3

Expression

Insect

Promoter

MT

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_EIF2A:1-427

Plasmid

#210938

Purpose

Insect expression of human EIF2A fragment M1 to T427. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2A:M1-T427 (EIF2A Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 4, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_COPB2:1-300

Plasmid

#210917

Purpose

Insect expression of human COPB2 fragment M1 to G300. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-G300 (COPB2 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_PRPF4:1-522

Plasmid

#210896

Purpose

Insect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PRPF4:M1-E522 (PRPF4 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR12:90-422

Plasmid

#210893

Purpose

Insect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:T90-G422 (WDR12 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR41:30-459

Plasmid

#210892

Purpose

Insect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:Q30-V459 (WDR41 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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