We narrowed to 116 results for: clostridium

1. pAF259

   Plasmid

   #105494

   Purpose

   pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

   Insert

   Bitlucopt
   

   Use

   Atc-dependent expression in c. difficile

   Promoter

   Ptet

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-ybbR-HIS-sfGFP-DocI

   Plasmid

   #58708

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   sfGFP-DocI
   

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIntegrase_11

   Plasmid

   #60582

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_11
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF256

   Plasmid

   #105496

   Purpose

   pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiT

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

   Insert

   HupA-SmBiT/LgBiT
   

   Use

   Atc-dependent expression in c. difficile

   Promoter

   Ptet

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF257

   Plasmid

   #105497

   Purpose

   pRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiT

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

   Insert

   SmBiT/HupA-LgBiT
   

   Use

   Atc-dependent expression in c. difficile

   Promoter

   Ptet

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAN334

   Plasmid

   #120814

   Purpose

   E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

   Depositor

   Craig Ellermeier, David S. Weiss

   Article

   Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

   Insert

   cyan fluorescent protein, codon-optimized for low GC organisms
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ybbR-HIS-CBM-CohI

   Plasmid

   #58709

   Purpose

   Probing molecule to perform single molecule force spectroscopy experiments

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   CBM-CohI
   

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Alanine 2 to Cysteine in CBM domain

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28 His6-mEGFP-D4H

   Plasmid

   #226373

   Purpose

   Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4H (pfoA Clostridium perfringens)
   

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28 His6-mEGFP-D4

   Plasmid

   #226372

   Purpose

   Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4 (pfoA Clostridium perfringens)
   

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRAN473

    Plasmid

    #120813

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAP118

    Plasmid

    #105495

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Promoter

    Ptet

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-ybbR-HIS-10FNIII(x4)-DocI

    Plasmid

    #58712

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    10FNIII(x4)-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-ybbR-HIS-Spec(x2)-DocI

    Plasmid

    #58713

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Spec(x2)-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUGW-UBC-EGFP-2A-BoNT-B (LC)

    Plasmid

    #185722

    Purpose

    Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    botulinum neurotoxin type B light chain
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-P5CR

    Plasmid

    #110295

    Purpose

    Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    Pyrroline-5-carboxylate Reductase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAN357

    Plasmid

    #120898

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

    Insert

    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVRa30_83

    Plasmid

    #49686

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF30_83
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

    Plasmid

    #169136

    Purpose

    E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    FIVAR (found in various architectures)
    

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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