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Plasmid#72982PurposeN-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)DepositorInsertN-terminal linker with pET-RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-HexHis
Plasmid#72983PurposeHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertPolyhistidine (HHHHHH) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL1
Plasmid#72985PurposeRBS_H1DepositorInsertH1 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL2
Plasmid#72986PurposeRBS_H2DepositorInsertH2 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL3
Plasmid#72987PurposeRBS_H3DepositorInsertH3 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-lacZ
Plasmid#72948PurposeEntry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloningDepositorInsertLevel 0 - Bioparts
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL80Uw-6
Plasmid#26236DepositorInsertGAL80 (GAL80 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp1
Plasmid#32148DepositorInserthsFlp1
UseTagsExpressionInsectMutationAspartic Acid at Amino Acid 5PromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBPp65ADZpUw
Plasmid#26234DepositorInsertp65AD-Zip (RELA Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pBPZpGAL4DBDUw
Plasmid#26233DepositorInsertZip-GAL4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS
Plasmid#196484PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterCMV (synthetase), Pol III tRNA promoter (tRNA)Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL4.2Uw-2
Plasmid#26227DepositorInsertGAL4 (GAL4 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS_v2
Plasmid#196485PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL4.1Uw
Plasmid#26226DepositorInsertGAL4 (GAL4 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV3FLAG_hGTPBP4
Plasmid#53974PurposeMammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.DepositorInsertGTPBP4 (GTPBP4 Human)
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBP_sgRNA
Plasmid#190128PurposeLevel 0 MoClo plasmid for Golden Gate cloning of sgRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
UseTagsExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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