We narrowed to 632 results for: weissman

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pJW1507

Plasmid

#62361

Purpose

pFA6a -mVenus-BirA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

c-terminal mVenus-BirA tagging

Use

Yeast c-terminal tagging vector

Tags

mVenus-BirA

Mutation

L232H mutation compared to standard mVenus

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG DTM

Plasmid

#185337

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 24-45, corresponding to the hairpi…

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG-DCUE

Plasmid

#185339

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 293-337 corresponding to the CUE d…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-mdm2/2702

Plasmid

#11492

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pGEM-human TCR zeta/2470

Plasmid

#11507

Depositor

Allan Weissman

Article

Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

Insert

TCR zeta (CD247 Human)

Use

General cloning

Mutation

n/a

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG27

Plasmid

#226742

Purpose

Expresses a reporter for a mitochondrial matrix protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

COX4 (COX4I1 Human)

Use

Lentiviral

Tags

GFP1-10

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1666

Plasmid

#112040

Purpose

CEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

None

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mE6-AP/2591a

Plasmid

#11468

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Tags

myc

Expression

Mammalian

Mutation

n/a

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pJW1663

Plasmid

#112037

Purpose

Gal-Estradiol transcription activation expression cassette marked with KanR

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

Gal DNA binding-estradiol binding-MSN2 activator

Expression

Yeast

Promoter

ADH1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2 Nsp3TMC111A

Plasmid

#204207

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

Cys to Ala in active site of PLpro

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Expression

Yeast

Promoter

PGK

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGM79

Plasmid

#220171

Purpose

CRISRPi guide RNA against MARCHF5

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

MARCHF5 (sgRNA) (MARCHF5 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-gp78/JM20

Plasmid

#13303

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2 Nsp3TM

Plasmid

#204206

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

none, from obtained clone

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd-4/2522

Plasmid

#11466

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 52-777

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-KG-UbcH5b/2443

Plasmid

#11477

Depositor

Allan Weissman

Insert

UbcH5b (UBE2D2 Human)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pJW1513

Plasmid

#62383

Purpose

pRS316-pTDH3-Su9-TagBFP

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

su9-tagBFP

Tags

Su9 presequence

Expression

Yeast

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTMB400_ZFcharm_Prnp_DPM

Plasmid

#220847

Purpose

AAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB399_ZFcharm_Prnp_SPM

Plasmid

#220846

Purpose

AAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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