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pGEX-Nedd-4/2522

Plasmid

#11466

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4-1 (Nedd4 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 52-777

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-UbcH5b/2443

Plasmid

#11477

Depositor

Allan Weissman

Insert

UbcH5b (UBE2D2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pCI-Neo-gp78/JM20

Plasmid

#13303

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1505

Plasmid

#62359

Purpose

RPL16a HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPL16A

Use

Yeast tagging

Tags

HA-TEV-AVI

Expression

Mutation

Promoter

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1513

Plasmid

#62383

Purpose

pRS316-pTDH3-Su9-TagBFP

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

su9-tagBFP

Use

Tags

Su9 presequence

Expression

Yeast

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1+ HA-hAO7

Plasmid

#137840

Purpose

N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-Nedd4-C / 2523 JM15

Plasmid

#13311

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 1-422 deleted

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pJW1518

Plasmid

#62388

Purpose

pFA6a -mCherry-BirA::HIS5

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

mCherry-BirA

Use

Yeast c-terminal tagging vector

Tags

mCherry-BirA

Expression

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-RI1-332-MYC-His6

Plasmid

#185351

Purpose

Mammalian expression of Ribophorin I

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ribophorin I (RPN1 Human)

Use

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

aa 1-332 only

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG_GI4

Plasmid

#111595

Purpose

GI Library Vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6p1 AO7RE

Plasmid

#137843

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG_GI2

Plasmid

#111593

Purpose

mU6-sgRNA GI Library vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG_GI3

Plasmid

#111594

Purpose

hU6-sgRNA GI Library vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-3-TRC8 513-664

Plasmid

#185352

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

TRC8 (RNF139 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 513-664 only

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HRD1 aa 236-444

Plasmid

#185349

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

HRD1/SYVN (SYVN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 236-444 only

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET3a-UBE2J1 1-283

Plasmid

#185348

Purpose

In vitro transcription and translation in bacterial system

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

UBE2J1 (UBE2J1 Human)

Use

Tags

Expression

Bacterial

Mutation

Cytoplasmic domain only, aa 1-283

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 AUP1 292-410

Plasmid

#185331

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1665

Plasmid

#112039

Purpose

Integrating plasmid for expression of ER localized mCherry at TRP1 locus

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ER-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge