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Plasmid#67856PurposeGFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residueDepositorInsertGFP fused to ELP (VPGAG) repeated 96 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-TEVP-cleavableELP
Plasmid#219402PurposeTobacco etch virus protease-responsive elastin-like polypeptide (ELP)DepositorInsertTEVP-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PIA900
Plasmid#104401PurposePolycistronic vector for expression of E. coli core RNAPDepositorInsertrpoA, B, C, and Z
UseE. coli expression vectorTagsencodes a C-terminally His-tagged rpoC which can …ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mpCHECK2
Plasmid#167576PurposeThe mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS37.13
Plasmid#106265PurposePolycistronic expression of ekivR, luc, esyn1 using P2ADepositorInsertekivR-P2A-luc-P2A-esyn1
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterTet-OnAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-DHFRHIS
Plasmid#63949PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-I48 ELP
Plasmid#67857PurposeGFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residuesDepositorInsertGFP fused to ELP (VPGIG) repeated 48 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEG_BB3_YL001
Plasmid#117825PurposeOverexpession plasmid of glycerol kinase (GUT1) in Y. lipolyticaDepositorInsertYlpTEF_YlGUT1_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-DHFRFLAG
Plasmid#63951PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-DHFRHIS
Plasmid#64003PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTS32.372
Plasmid#106262PurposePolycistronic expression of luc, ekivR, esyn1 using P2ADepositorInsertluc-P2A-ekivR-P2A-esyn1
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterTet-OnAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
rFIXX-bp chain A (IRESneo)
Plasmid#82450PurposeChain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.DepositorInsertrFIXX-bp chain A
UseTagsPolyhistidineExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-DHFRFLAG
Plasmid#64006PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-DHFRHIS
Plasmid#63985PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-DHFRFLAG
Plasmid#63987PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-DHFRCYD
Plasmid#64005PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCYDExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-DHFRFLAG
Plasmid#63969PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCYP44
Plasmid#212877PurposeExpresses the Saccharopolyspora shandongensis LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP35
Plasmid#212868PurposeExpresses the Saccharopolyspora antimicrobica LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP36
Plasmid#212869PurposeExpresses the Saccharopolyspora elongata LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWBhis03
Plasmid#188556PurposeBinary vector with MpIGPDm as a selective marker in Marchantia polymorpha (igpd mutants)DepositorInsertMpIGPDm
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Akaluc VLP
Plasmid#182212PurposeYIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-Akaluc
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIAGO-AceF1
Plasmid#114461PurposeExpression of late polyene biosynthetic genes in streptomycetesDepositorInsertAceDI + AceDII + AceN + AceM
UseTagsExpressionBacterialMutationPromoterermEAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS38.3
Plasmid#106266PurposePolycistronic expression of ekivR, esyn1, luc using P2ADepositorInsertekivR-P2A-esyn1-P2A-luc
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterTet-OnAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBK-CMV Pig12
Plasmid#16506DepositorInsertPig12 (PTGES Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
V96 ELP
Plasmid#68392PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residueDepositorInsertV96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tubp-Gal80 in pCaSpeR4
Plasmid#17748DepositorInsertGAL80 (GAL80 Budding Yeast)
UseTagsSV40 polyA and Tubulin 1alpha promoterExpressionInsectMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
V72 ELP
Plasmid#68938PurposeElastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residueDepositorInsertV72 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_MFE1
Plasmid#84612PurposeIntroduces DSB at MFE1 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only