Addgene

We narrowed to 4,899 results for: puc

Showing: 2451 - 2500 of 4899 results

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pHelper_AcCAST

Plasmid

#127923

Purpose

Expresses AcCAST and a crRNA targeting PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_D17

Plasmid

#84611

Purpose

Introduces DSB at D17 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pT1UciMP

Plasmid

#62215

Purpose

UAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

UAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_A08

Plasmid

#84610

Purpose

Introduces DSB at A08 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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KIF5A_Halo_C_allele

Plasmid

#178163

Purpose

Donor vector for endogenous tagging of human KIF5A at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (KIF5A Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGGB003

Plasmid

#48821

Purpose

Provides a default dummy as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

B-Dummy

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-miRFP670-pA_U6-BbsI (pE059)

Plasmid

#149353

Purpose

Mammalian expression vector for miRFP670

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

miRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_XPR2

Plasmid

#84609

Purpose

Introduces DSB at XPR2 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK-EL-03

Plasmid

#125762

Purpose

Endlinker for tRNA-gRNA system when using only three guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRyl_AXP

Plasmid

#84608

Purpose

Introduces DSB at AXP locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT13

Plasmid

#200993

Purpose

pLv1-pUC18R6KT-miniTn7-Tc, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT14

Plasmid

#201015

Purpose

pLv1-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT12

Plasmid

#200992

Purpose

pLv1-pUC18R6KT-miniTn7-Km, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM106B_Halo_C_allele

Plasmid

#178173

Purpose

Donor vector for endogenous tagging of human TMEM106B at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (TMEM106B Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaU6-esgRNA

Plasmid

#115630

Purpose

expression esgRNA in wheat protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

TaU6p-esgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAllet

Plasmid

#202560

Purpose

E. coli cloning vector with large MCS (50+ unique restriction sites)

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGD002

Plasmid

#48834

Purpose

Provides a default dummy as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

D-Dummy

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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MTST6

Plasmid

#86603

Purpose

Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.

Depositor

Mary Ann Moran

Article

Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1

Insert

MTST6

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pZD-CJ1111

Plasmid

#175058

Purpose

rbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGA006

Plasmid

#48816

Purpose

Provides the UBQ10 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ111

Plasmid

#175052

Purpose

rbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TC646

Plasmid

#164892

Purpose

Pdpy30::loxP::mcherry::let-858terminator::loxP::GFP

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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EZH2_N_FS_v2_Donor

Plasmid

#79901

Purpose

N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Expression

Mutation

Promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7xKS

Plasmid

#117393

Purpose

Tn7 tagging vector with a LacI-controlled kill switch counterselection system

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETSIX

Plasmid

#74104

Purpose

mCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

tetA(C)

Use

Conjugative bacterial vector

Tags

Expression

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM34DEST

Plasmid

#74756

Purpose

Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
601-145-8X

Plasmid

#158620

Purpose

DNA fragment production for nucleosome core particle preparation

Depositor

Curt Davey

Article

Vasudevan et al J Mol Biol. 2010 Oct 15;403(1):1-10. doi

Type

Empty backbone

Use

Bacterial vector for dna production

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT21

Plasmid

#200995

Purpose

pLv2-pUC18R6KT-miniTn7-Gm, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT22

Plasmid

#200996

Purpose

pLv2-pUC18R6KT-miniTn7-Km, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT23

Plasmid

#200997

Purpose

pLv2-pUC18R6KT-miniTn7-Tc, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT24

Plasmid

#200998

Purpose

pLv2-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT20

Plasmid

#200990

Purpose

pL0V-Lv2-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE009

Plasmid

#48841

Purpose

Provides the UBQ10 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
VCP_Halo_C_allele

Plasmid

#178132

Purpose

Donor vector for endogenous tagging of human VCP at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (VCP Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7xTS

Plasmid

#117389

Purpose

Tn7 tagging vector with temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0245

Plasmid

#162284

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQDKN0729

Plasmid

#162316

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK-EL-01

Plasmid

#125760

Purpose

Endlinker for tRNA-gRNA system when using only one guide

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK-EL-04

Plasmid

#125763

Purpose

Endlinker for tRNA-gRNA system when using only four guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK-EL-05

Plasmid

#125764

Purpose

Endlinker for tRNA-gRNA system when using only five guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK-EL-02

Plasmid

#125761

Purpose

Endlinker for tRNA-gRNA system when using only two guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGA012

Plasmid

#48818

Purpose

Provides the RPS5A promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

RPS5A promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p214_LTJ_1kbCD90.1Template

Plasmid

#82667

Purpose

1kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOsU3-esgRNA

Plasmid

#115629

Purpose

expression esgRNA in rice protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

OsU3p-esgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK398

Plasmid

#114714

Purpose

mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
VAPB_Halo_C_allele

Plasmid

#178174

Purpose

Donor vector for endogenous tagging of human VAPB at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (VAPB Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT1QciMP

Plasmid

#62216

Purpose

QUAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

QUAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-302 (attR4-attR2)

Plasmid

#161894

Purpose

Multisite Gateway (attR4-attR2) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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