We narrowed to 9,709 results for: Gnas

1. pLVX-CMV-Galpha-oA ONE-GO

   Plasmid

   #189732

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-oA ONE-GO
   GNAO (Gnao1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   I250 (silent mutation in YFP)

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Cx36-GCaMP

   Plasmid

   #123604

   Purpose

   Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

   Depositor

   John O'Brien

   Article

   Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

   Insert

   Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
   

   Use

   Tags

   GCaMP3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc

   Plasmid

   #223559

   Purpose

   Alias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

   Depositor

   Mikel Garcia-Marcos

   Article

   Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

   Insert

   YFP(Venus) - KRas4b - IRES* - human GINIP - linker(GGGS) - Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGEX-SUMO1

   Plasmid

   #185637

   Purpose

   expression of GST-tagged SUMO1 in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

   Insert

   small ubiquitin-like modifier 1 (SUMO1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCLc-MND-A0201-SABR-Backbone

   Plasmid

   #119050

   Purpose

   3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

   Depositor

   David Baltimore

   Article

   Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MND

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCCLc-MND-A0201-Mart1-SABR

   Plasmid

   #119052

   Purpose

   3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

   Depositor

   David Baltimore

   Article

   Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

   Insert

   A0201-Mart1-SABR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MND

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-mito-LAR-GECO1.2

   Plasmid

   #61245

   Purpose

   Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1.2
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1.2: N45I/A47R/E1…

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-CMV-Galpha-oB ONE-GO

   Plasmid

   #189733

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-oB ONE-GO
   GNAO (Gnao1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid

    #207150

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI3 (GNAI3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-eEF2K

    Plasmid

    #110160

    Purpose

    Expression of human eEF2K in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p667-UBC-NRF1-V5-miniTurbo

    Plasmid

    #207810

    Purpose

    C-terminal tagged NRF1 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    NRF1-V5-miniTurbo (NFE2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-miniTurbo-V5-NRF1

    Plasmid

    #207809

    Purpose

    N-terminal tagged NRF1 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-NRF1 (NFE2L1 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pg-EGFP-HIF-2alpha

    Plasmid

    #87207

    Purpose

    N-terminally EGFP-tagged human HIF-2a/EPAS

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-2a (EPAS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q868E

    Promoter

    CMV

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-NLS-CBGreen-FIRE-puro

    Plasmid

    #139195

    Purpose

    Luminescent reporter of ERK activity

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBG99
    

    Use

    Luciferase and Retroviral

    Tags

    Fra1 PEST domain fragment (FIRE) and nuclear loca…

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR221-FGFR4

    Plasmid

    #60531

    Purpose

    Entry vector for human FGFR4

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    c2134a P712T

    Promoter

    NA

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCCLc-MND-B2705-SABR-Backbone

    Plasmid

    #119051

    Purpose

    3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ER-CanlonicSF

    Plasmid

    #178343

    Purpose

    To explore endoplasmic reticulum lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    ER-CanlonicSF
    

    Use

    Tags

    ER Retention Signal, ER Signal Exon 1, and ER Sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCCLc-MND-B2705-KK10-SABR

    Plasmid

    #119053

    Purpose

    3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    B2705-KK10-SABR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MND

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge