We narrowed to 1,829 results for: pb

1. pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78307

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADflluw
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

   Plasmid

   #233443

   Purpose

   PiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

   Plasmid

   #233446

   Purpose

   PiggyBac donor plasmid to overexpress ADAR2dd-PCP

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2dd-PCP-T2A-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

   Plasmid

   #233442

   Purpose

   PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

   Plasmid

   #233441

   Purpose

   PiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP

   Plasmid

   #233444

   Purpose

   PiggyBac donor plasmid to overexpress ADAR2dd-MCP

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2dd-MCP-T2A-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

   Plasmid

   #233413

   Purpose

   LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   LIDARSensor(mCherry->Casp9-2A-GFP)-hygro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine

   Plasmid

   #204732

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA

   Plasmid

   #140224

   Purpose

   Constitutive PGK driven expression of intein with miniVPR for 3-way split activator

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   C-intein with miniVPR
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204723

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

    Plasmid

    #184951

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Patrick Hsu, Lacra Bintu, Ami Bhatt, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

    Plasmid

    #168107

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    Cas9-P2A-mCherry
    

    Use

    CRISPR and Cre/Lox

    Tags

    HA

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

    Plasmid

    #61255

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

    Plasmid

    #184952

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Patrick Hsu, Lacra Bintu, Ami Bhatt, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

    Plasmid

    #172066

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

    Plasmid

    #140221

    Purpose

    Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

    Plasmid

    #172065

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

    Plasmid

    #158542

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 1-480, C-terminal deletion, F188D, W2…

    Promoter

    CMV

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5

    Plasmid

    #61257

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge