We narrowed to 1,241 results for: post

1. hETFDH CRISPR_1

   Plasmid

   #232309

   Purpose

   Human ETFDH Gene Knockout (gRNA 1)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEtfdh CRISPR_2

   Plasmid

   #232313

   Purpose

   Mouse Etfdh Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh-targeting gRNA (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEtfdh CRISPR_1

   Plasmid

   #232312

   Purpose

   Mouse Etfdh Gene Knockout (gRNA 1)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh-targeting gRNA (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. hETFDH CRISPR_2

   Plasmid

   #232310

   Purpose

   Human ETFDH Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

   Plasmid

   #196191

   Purpose

   Tagging human SOX2 gene with P2A-EGFP at the C-terminus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
   

   Use

   Homology repair donor

   Tags

   Expression

   Mutation

   G2913A mutation to replace the guide cutting site…

   Promoter

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFPC2-Gephyrin P1

   Plasmid

   #68815

   Purpose

   expression of fluorescent tagged Geph in mammalian cells

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyri…

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

   Plasmid

   #145027

   Purpose

   Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-Tq-Ca-FLITS
   

   Use

   Lentiviral

   Tags

   3xnls-Tq-Ca-FLITS and H2B-Maroon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR3-FLAG-Gephyrin P1

   Plasmid

   #68816

   Purpose

   Flag tagged Geph mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pIRESh-GFPII-SM-Dlk1

    Plasmid

    #108932

    Purpose

    GFP-tagged vector expressing full length isoform of Dlk1

    Depositor

    Anne Ferguson-Smith

    Article

    Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

    Insert

    Dlk1 (Dlk1 Mouse)
    

    Use

    Tags

    GFPII

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid

    #85072

    Purpose

    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    

    Use

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mouse Alpha2 delta-3 pMT2

    Plasmid

    #58727

    Purpose

    expression of mouse Alpha2 delta-3 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-3 (Cacna2d3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mouse Alpha2 delta-3 HA pMT2

    Plasmid

    #58728

    Purpose

    expression of HA tagged mouse Alpha2 delta-3 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-3 (Cacna2d3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZE21/UBP1/ClpS

    Plasmid

    #98566

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    In a synthetic operon downstream of UBP1 and Remo…

    Promoter

    Same as UBP1 (operon) and Tet promoter (aTc induc…

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFPC2-gephyrin S268A/270A

    Plasmid

    #199608

    Purpose

    Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S268A/S270A

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCPF101-pfQC-WT

    Plasmid

    #184877

    Purpose

    expresses soluble plasmodium falciparum QC protein with a histag

    Depositor

    Ferenc Scheeren

    Article

    Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    pfQC-wt
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR3-FLAG-Gephyrin E

    Plasmid

    #68819

    Purpose

    Flag tagged mammalian expression of E domain

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    internal EcoRI site has silent mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. MBP-rPKL WT

    Plasmid

    #107156

    Purpose

    MBP-rPKL WT for production and purification of rat PKL WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-Mm_MyoGEF(WT)

    Plasmid

    #136325

    Purpose

    Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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