We narrowed to 6,573 results for: tac

1. pGEX-parkin W403A

   Plasmid

   #45974

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin W403A (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-parkin C431S

   Plasmid

   #45976

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin C431S (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T2-Snf1-cat

   Plasmid

   #52683

   Purpose

   expression of the Snf1 kinase domain in bacteria

   Depositor

   Benjamin Turk

   Article

   Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

   Insert

   SNF1 (SNF1 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes kinase domain only, aa 41-315

   Promoter

   tac

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGEX-parkin F463A

   Plasmid

   #45975

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin F463A (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   tac

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AEB-No-aptamer-controlluciferase

   Plasmid

   #63853

   Purpose

   This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.

   Depositor

   Howard Salis

   Article

   Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

   Insert

   No-aptamer control mRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tac

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GST-PRMT9 L182A/D183A/I184A/G185A

   Plasmid

   #67608

   Purpose

   bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   PRMT9 (PRMT9 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   catalytic mutant LDIG(182-185)AAAA

   Promoter

   tac

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGEX4T-Mst1 SARAH

   Plasmid

   #84297

   Purpose

   Expresses Mst1 SARAH domain for interaction studies.

   Depositor

   Jonathan Chernoff

   Article

   Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

   Insert

   Mst1 (STK4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pGEX-parkin F146A

   Plasmid

   #45973

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin F146A (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Mishler2014-No-aptamer-controlluciferase-pUC19

    Plasmid

    #63856

    Purpose

    This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    No-aptamer control mRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pGEX4T-1_OCT4_T234A_S235A

    Plasmid

    #40634

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV cleavage, and Thrombin cleavage

    Expression

    Mammalian

    Mutation

    T234A_S235A

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

12. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

    Plasmid

    #222990

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGex-6p2_NSD2

    Plasmid

    #221376

    Purpose

    Expresses NSD2 in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Histone-lysine N-methyltransferase NSD2 (NSD2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGex-6p2_ATRX_K1033R

    Plasmid

    #221378

    Purpose

    Expresses ATRX_K1033R in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Transcriptional regulator ATRX (ATRX Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed lysine 1033 to arginine

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGex-6p2_FANCM_K819R

    Plasmid

    #221380

    Purpose

    Expresses FANCM_K819R in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Fanconi anemia group M protein (FANCM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed lysine 819 to arginine

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PABPC1_544-626_pGEX6P1

    Plasmid

    #209790

    Purpose

    Bacterial expression of PABPC1 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

    Insert

    Mlle domain of PABPC1 (PABPC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 544-626 only

    Promoter

    tac

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-2T_E30_VP1

    Plasmid

    #202070

    Purpose

    Bacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    E30 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-2T_CVB1_VP1

    Plasmid

    #202069

    Purpose

    Bacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    CVB1 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-2T_CVA4_VP1

    Plasmid

    #202068

    Purpose

    Bacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    CVA4 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid

    #196973

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GFP and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge