Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

171,784 results

Showing: 9981 - 10000 of 171784 results

  7. pCAGGS-FuG-E

    Plasmid
    #67509
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci Methods. 2014 Apr 30;227:151-
    Insert
    Fusion Glycoprotein type E
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Promoter
    CAGGS
    Available Since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67509-plasmid-map-sequence-id-118281

    Enlarge

  8. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

    Enlarge

  9. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

    Enlarge

  10. pFastbac1 Nterminal GST

    Plasmid
    #128058
    Purpose
    Baculovirus expression vector with N terminal GST
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    GST
    Expression
    Insect
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128058-plasmid-map-sequence-id-255102

    Enlarge

  14. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

    Enlarge

  19. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid
    #64921
    Purpose
    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPE1 (PCBP1 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64921-plasmid-map-sequence-id-109667

    Enlarge

Showing: 9981 - 10000 of 171784 results